PTK7 Protein, Rat (HEK293, His)

The PTK7 protein is predicted to mediate cell adhesion molecule binding and play critical roles in a variety of cellular processes, including actin cytoskeleton reorganization and regulation of the canonical Wnt signaling pathway. It affects animal organ development, axis elongation, and epithelial morphogenesis. PTK7 Protein, Rat (HEK293, His) is the recombinant rat-derived PTK7 protein, expressed by HEK293 , with C-10*His labeled tag. The total length of PTK7 Protein, Rat (HEK293, His) is 673 a.a., with molecular weight of 95-130 kDa.

The PTK7 protein is anticipated to play a crucial role in mediating cell adhesion molecule binding activity. Its involvement is predicted in diverse cellular processes, including the reorganization of the actin cytoskeleton, cellular response to retinoic acid, and positive regulation of the canonical Wnt signaling pathway. Additionally, it is expected to exert influence upstream of or within essential processes such as animal organ development, axis elongation, and epithelial morphogenesis. The predicted subcellular localization of PTK7 is in the membrane, with anticipated activity at cell-cell junctions and the plasma membrane. Notably, PTK7 demonstrates biased expression, with elevated levels observed in the lung (RPKM 114.4), uterus (RPKM 97.1), and eight other tissues, indicating its potential significance in these physiological contexts. [information derived from Alliance of Genome Resources, April 2022]

Measured by its binding ability in a functional ELISA. Recombinant Rat PTK7/CCK4 Fc Chimera binds Biotinylated Recombinant Mouse Wnt-3a. The ED₅₀ for this effect is 8.203 μg/mL.

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  Measured by its binding ability in a functional ELISA. Recombinant Rat PTK7/CCK4 Fc Chimera binds Biotinylated Recombinant Mouse Wnt-3a. The ED₅₀ for this effect is 8.203 μg/mL.

Rat

HEK293

C-10*His

NP_001382670.1 (A23-Q695)

301242  [NCBI]

AIVFIKEPSSQDALQGRRALLRCEVEAPDPVHVYWLLNGVPVQDTERRFAQGSSLSFAAVDRLQDSGAFQCVARDNITGEEARSANASFNIKWIEAGPVVLKHPASAAEIQPQTQVTLRCHIDGHPRPTYQWFRDGTPLSDDQSTHTVSSKERNLTLRPASPEHSGLYSCCAHNAFGQACSSQNFTLSIADESFARVLLAPQDVVVARNEEAMFHCQFSAQPPPSLQWVFEDETPITNRSRPPHLRKAMVFANGSLLLTQVRPRNAGVYRCIGQGQRGPPIVLEATLHLAEIEDMLPLEPRVFIAGGEERVACPAPQGLPTPSVWWEHAGVRLPAHGRVHQKGLELVFATIAESDAGVYTCHAANLAGQRRQDVNITVATVPTWLRKPQDSQLEEGKPGYLHCLTQATPKPTVIWYRNQMLISEDSRFEVSKNGTLRINSVEVYDGTVYRCVSSTPAGSIEAQARVQVLEKLKFTPPPQPQQCMEFDKEATVPCSATGREKPTVKWVRADGSSLPEWVTDNAGTLHFARVTRDDAGNYTCIASNEPQGQIRAHVQLTVAVFITFKVEPERTTVYQGHTALLRCEAQGDPKPLIQWKGKDRILDPTKLGPRMHIFQNGSLVIHDVAPEDSGSYTCIAGNSCNIRHTEAPLLVVDKPLMEDSEGPGSPPPYKMIQ

95-130 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.