PTK7 Protein, Rat (HEK293, His)
Based on 1 publication(s) in Google Scholar
The PTK7 protein is predicted to mediate cell adhesion molecule binding and play critical roles in a variety of cellular processes, including actin cytoskeleton reorganization and regulation of the canonical Wnt signaling pathway. It affects animal organ development, axis elongation, and epithelial morphogenesis. PTK7 Protein, Rat (HEK293, His) is the recombinant rat-derived PTK7 protein, expressed by HEK293 , with C-10*His labeled tag.
- Species: Rat
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The PTK7 protein is predicted to mediate cell adhesion molecule binding and play critical roles in a variety of cellular processes, including actin cytoskeleton reorganization and regulation of the canonical Wnt signaling pathway. It affects animal organ development, axis elongation, and epithelial morphogenesis. PTK7 Protein, Rat (HEK293, His) is the recombinant rat-derived PTK7 protein, expressed by HEK293 , with C-10*His labeled tag.
Background
The PTK7 protein is anticipated to play a crucial role in mediating cell adhesion molecule binding activity. Its involvement is predicted in diverse cellular processes, including the reorganization of the actin cytoskeleton, cellular response to retinoic acid, and positive regulation of the canonical Wnt signaling pathway. Additionally, it is expected to exert influence upstream of or within essential processes such as animal organ development, axis elongation, and epithelial morphogenesis. The predicted subcellular localization of PTK7 is in the membrane, with anticipated activity at cell-cell junctions and the plasma membrane. Notably, PTK7 demonstrates biased expression, with elevated levels observed in the lung (RPKM 114.4), uterus (RPKM 97.1), and eight other tissues, indicating its potential significance in these physiological contexts. [information derived from Alliance of Genome Resources, April 2022]
Verified Bioactivity
1.This product does not contain protein kinase domain.
2.Measured by its binding ability in a functional ELISA. Recombinant Rat PTK7/CCK4 binds Biotinylated Recombinant Mouse Wnt-3a. The ED50 for this effect is 8.203 μg/mL.
Publications (1)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
An aptamer-drug conjugate for promising cancer therapy with comprehensive evaluation from rodents to non-human primates. [Abstract]2025 Sep 24;10(1):316. PMID: 40987771
Technical Parameters
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Species Rat
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Source HEK293
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Tag C-10*His
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Accession
A0A8I6AKN3/NP_001382670.1 (A23-Q695)
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Molecular Construction
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N-term
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PTK7 (A23-Q695)
Accession # A0A8I6AKN3/NP_001382670.1 -
10*His
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C-term
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Protein Length
Partial
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Synonyms
PTK7; PTK7 Protein Tyrosine Kinase 7; Protein Tyrosine Kinase 7 (Inactive); Tyrosine-Protein Kinase-Like 7; CCK4; CCK-4; Colon Carcinoma Kinase 4; Protein Tyrosine Kinase 7; Inactive Tyrosine-Protein Kinase 7; Protein-Tyrosine Kinase 7; Pseudo Tyrosine Ki
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AA Sequence
AIVFIKEPSSQDALQGRRALLRCEVEAPDPVHVYWLLNGVPVQDTERRFAQGSSLSFAAVDRLQDSGAFQCVARDNITGEEARSANASFNIKWIEAGPVVLKHPASAAEIQPQTQVTLRCHIDGHPRPTYQWFRDGTPLSDDQSTHTVSSKERNLTLRPASPEHSGLYSCCAHNAFGQACSSQNFTLSIADESFARVLLAPQDVVVARNEEAMFHCQFSAQPPPSLQWVFEDETPITNRSRPPHLRKAMVFANGSLLLTQVRPRNAGVYRCIGQGQRGPPIVLEATLHLAEIEDMLPLEPRVFIAGGEERVACPAPQGLPTPSVWWEHAGVRLPAHGRVHQKGLELVFATIAESDAGVYTCHAANLAGQRRQDVNITVATVPTWLRKPQDSQLEEGKPGYLHCLTQATPKPTVIWYRNQMLISEDSRFEVSKNGTLRINSVEVYDGTVYRCVSSTPAGSIEAQARVQVLEKLKFTPPPQPQQCMEFDKEATVPCSATGREKPTVKWVRADGSSLPEWVTDNAGTLHFARVTRDDAGNYTCIASNEPQGQIRAHVQLTVAVFITFKVEPERTTVYQGHTALLRCEAQGDPKPLIQWKGKDRILDPTKLGPRMHIFQNGSLVIHDVAPEDSGSYTCIAGNSCNIRHTEAPLLVVDKPLMEDSEGPGSPPPYKMIQ
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Molecular Weight
Approximately 95-116 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (240 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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