PVR/CD155 Protein, Rat (HEK293, His)
Based on 1 Customer Validation
The PVR/CD155 protein, encoded by the PVR gene, acts as a receptor for poliovirus. It is involved in the regulation of heterophilic and homophilic cell adhesion, as well as leukocyte-mediated cytotoxicity. PVR/CD155 Protein, Rat (HEK293, His) is the recombinant rat-derived PVR/CD155 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Rat
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The PVR/CD155 protein, encoded by the PVR gene, acts as a receptor for poliovirus. It is involved in the regulation of heterophilic and homophilic cell adhesion, as well as leukocyte-mediated cytotoxicity. PVR/CD155 Protein, Rat (HEK293, His) is the recombinant rat-derived PVR/CD155 protein, expressed by HEK293 , with C-His labeled tag.
Background
The PVR gene encodes the poliovirus receptor (PVR), also known as CD155, which functions as a virus receptor. PVR is predicted to be involved in heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules, homophilic cell adhesion via plasma membrane adhesion molecules, and positive regulation of leukocyte-mediated cytotoxicity. It is anticipated to act upstream of or within processes such as cell migration and cell-cell adhesion, and is predicted to be located in the cell surface and plasma membrane, serving as an integral component of the membrane with activity in adherens junctions. The orthologous counterpart in humans is PVR (PVR cell adhesion molecule). With biased expression observed in the lung (RPKM 65.2), heart (RPKM 42.8), and nine other tissues, PVR/CD155 plays a vital role in mediating cell adhesion and potentially influencing immune responses.
Verified Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized rat PVR/CD155 at 20 μg/mL (100 μL/well) can bind Rat DNAM-1/CD226. The ED50 for this effect is 796.7 ng/mL.
Technical Parameters
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Species Rat
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Source HEK293
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Tag C-His
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Accession
NP_058772 (E34-G352)
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Molecular Construction
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N-term
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PVR (E34-G352)
Accession # NP_058772 -
His
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C-term
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Protein Length
Partial
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Synonyms
PVR; Tage4; Prev. PVS; Nectin-Like Protein 5; Poliovirus Receptor; Poliovirus Receptor, Isoform CRA_c; Necl-5; Nectin-Like 5; CD155; CD155 Antigen; NECL5; NECL-5; PVR Cell Adhesion Molecule; HVED
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AA Sequence
EIAVQVLSNSTGFLGGSTVLHCSLASKDNVTITQLTWMKRDPDGSHPSVAVFHPKKGPSISDPERVKFLVAKVYEDLRNASLAISNLRVEDEGIYECQIATFPTGSKSANVWLKVFARPKNTAEALEPSPTLMPQDVAKCISADGHPPGRITWSSNVNGSYREMKETGSQPGTTTVISYLSMVPSSQADGTNITCTVEHESFQEPDQQPLILSLPYPPEVSISGYEGNWYIGLTNVNLTCEARSKPPPTNYSWSTATGPLPNSTHFQENGSHLLISTVDDLNNTIFVCKAINALGSGQGQVTILVKEASEILPPKTSLG
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Molecular Weight
Approximately 55-65 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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