RAD51 Protein, Human (His)

RAD51 Protein, Human (His) is the recombinant human-derived RAD51, expressed by E. coli , with N-6*His labeled tag. ,

Rad51 plays a crucial role in homologous strand exchange, a key step in DNA repair via homologous recombination (HR). It binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments essential for homology search and strand exchange. Rad51 catalyzes the recognition of homology and strand exchange between homologous DNA partners, forming a joint molecule between broken DNA and the repair template. During replication stress, Rad51 is recruited to resolve stalled replication forks. Additionally, Rad51 is part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C, which is thought to function in DNA repair through HR. Under oxidative stress conditions, Rad51, along with RAD51C and XRCC3, regulates mitochondrial DNA copy number. Rad51 is also involved in interstrand cross-link repair.

Human

E. coli

N-6*His

Q06609-1 (A2-D339)

5888

Full Length of Isoform-1 Mature Protein

AMQMQLEANADTSVEEESFGPQPISRLEQCGINANDVKKLEEAGFHTVEAVAYAPKKELINIKGISEAKADKILAEAAKLVPMGFTTATEFHQRRSEIIQITTGSKELDKLLQGGIETGSITEMFGEFRTGKTQICHTLAVTCQLPIDRGGGEGKAMYIDTEGTFRPERLLAVAERYGLSGSDVLDNVAYARAFNTDHQTQLLYQASAMMVESRYALLIVDSATALYRTDYSGRGELSARQMHLARFLRMLLRLADEFGVAVVITNQVVAQVDGAAMFAADPKKPIGGNIIAHASTTRLYLRKGRGETRICKIYDSPCLPEAEAMFAINADGVGDAKD

42 kDa

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH 8.0 or PBS, 6% Trehalose, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.