RAD51 Protein, Human (His)
Based on 1 publication(s) in Google Scholar
RAD51 Protein, Human (His) is the recombinant human-derived RAD51, expressed by E. coli , with N-6*His labeled tag. ,
- Species: Human
- Source: E. coli
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
RAD51 Protein, Human (His) is the recombinant human-derived RAD51, expressed by E. coli , with N-6*His labeled tag. ,
Background
Rad51 plays a crucial role in homologous strand exchange, a key step in DNA repair via homologous recombination (HR). It binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments essential for homology search and strand exchange. Rad51 catalyzes the recognition of homology and strand exchange between homologous DNA partners, forming a joint molecule between broken DNA and the repair template. During replication stress, Rad51 is recruited to resolve stalled replication forks. Additionally, Rad51 is part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C, which is thought to function in DNA repair through HR. Under oxidative stress conditions, Rad51, along with RAD51C and XRCC3, regulates mitochondrial DNA copy number. Rad51 is also involved in interstrand cross-link repair.
Publications (1)
-
Journal Impact Factor
-
Most Recent
Technical Parameters
-
Species Human
-
Source E. coli
-
Tag N-6*His
-
Accession
Q06609-1 (A2-D339)
-
Gene ID5888
-
Protein Length
Full Length of Isoform-1
-
Synonyms
RAD51; RAD51 Homolog A; Prev. RAD51A; RAD51 Homolog (RecA Homolog, E. Coli) (S. Cerevisiae); Prev. RECA; RAD51 (S. Cerevisiae) Homolog (E Coli RecA Homolog); HsT16930; RAD51 Homolog (S. Cerevisiae); HsRad51; RecA, E. Coli, Homolog Of; BRCC5; Recombination
-
AA Sequence
AMQMQLEANADTSVEEESFGPQPISRLEQCGINANDVKKLEEAGFHTVEAVAYAPKKELINIKGISEAKADKILAEAAKLVPMGFTTATEFHQRRSEIIQITTGSKELDKLLQGGIETGSITEMFGEFRTGKTQICHTLAVTCQLPIDRGGGEGKAMYIDTEGTFRPERLLAVAERYGLSGSDVLDNVAYARAFNTDHQTQLLYQASAMMVESRYALLIVDSATALYRTDYSGRGELSARQMHLARFLRMLLRLADEFGVAVVITNQVVAQVDGAAMFAADPKKPIGGNIIAHASTTRLYLRKGRGETRICKIYDSPCLPEAEAMFAINADGVGDAKD
-
Predicted Molecular Mass
40.9 kDa
-
Molecular Weight
Approximately 42 kDa, based on SDS-PAGE under reducing conditions.
-
Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH 8.0 or PBS, 6% Trehalose, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
-
Data Sheet (237 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
