Raptin/Reticulocalbin-2 Protein, Human (His)

Raptin a sleep-inducible hypothalamic protein hormone that suppresses obesity. Raptin is cleaved from reticulocalbin-2 (RCN2). Raptin release is timed by the circuit from vasopressin-expressing neurons in the suprachiasmatic nucleus to RCN2-positive neurons in the paraventricular nucleus. Raptin/Reticulocalbin-2 Protein, Human (His) is a recombinant Raptin protein expressed by E.coli with an N-6*His tag.

Sleep deficiency is associated with obesity. Raptin is a sleep-inducible hypothalamic protein hormone in humans and mice that suppresses obesity. Raptin levels peak during sleep, which is blunted by sleep deficiency. Raptin binds to glutamate metabotropic receptor 3 (GRM3) in neurons of the hypothalamus and stomach to inhibit appetite and gastric emptying, respectively. Raptin represents a new rhythmic protein hormone that suppresses appetite, providing new insights into body weight control^[1].

Human

E. coli

N-6*His

Q14257-1 (S28-P249)

5955

Partial

Approximately 38 kDa, based on SDS-PAGE under reducing conditions.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Xie LQ, et al. Raptin, a sleep-induced hypothalamic hormone, suppresses appetite and obesity. Cell Res. 2025 Mar;35(3):165-185.  [Content Brief]