Raptin/Reticulocalbin-2 Protein, Human (His)
Based on 1 Customer Validation
Raptin a sleep-inducible hypothalamic protein hormone that suppresses obesity. Raptin is cleaved from reticulocalbin-2 (RCN2). Raptin release is timed by the circuit from vasopressin-expressing neurons in the suprachiasmatic nucleus to RCN2-positive neurons in the paraventricular nucleus. Raptin/Reticulocalbin-2 Protein, Human (His) is a recombinant Raptin protein expressed by E.coli with an N-6*His tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Raptin a sleep-inducible hypothalamic protein hormone that suppresses obesity. Raptin is cleaved from reticulocalbin-2 (RCN2). Raptin release is timed by the circuit from vasopressin-expressing neurons in the suprachiasmatic nucleus to RCN2-positive neurons in the paraventricular nucleus. Raptin/Reticulocalbin-2 Protein, Human (His) is a recombinant Raptin protein expressed by E.coli with an N-6*His tag.
Sleep deficiency is associated with obesity. Raptin is a sleep-inducible hypothalamic protein hormone in humans and mice that suppresses obesity. Raptin levels peak during sleep, which is blunted by sleep deficiency. Raptin binds to glutamate metabotropic receptor 3 (GRM3) in neurons of the hypothalamus and stomach to inhibit appetite and gastric emptying, respectively. Raptin represents a new rhythmic protein hormone that suppresses appetite, providing new insights into body weight control[1].
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His
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Accession
Q14257-1 (L28-P249)
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Gene ID5955
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Molecular Construction
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N-term
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6*His
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Raptin (L28-P249)
Accession # Q14257 -
C-term
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Protein Length
Partial
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Synonyms
RCN2; Reticulocalbin 2, EF-Hand Calcium Binding Domain (Endoplasmic Reticulum Calcium-Binding Protein, 55kD); Reticulocalbin 2; Calcium-Binding Protein ERC-55; ERC55; E6-Binding Protein; E6BP; Reticulocalbin-2; ERC-55; Reticulocalbin 2, EF-Hand Calcium Bi
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AA Sequence
LHYPLGERRSDYDREALLGVQEDVDEYVKLGHEEQQKRLQAIIKKIDLDSDGFLTESELSSWIQMSFKHYAMQEAKQQFVEYDKNSDDTVTWDEYNIQMYDRVIDFDENTALDDAEEESFRKLHLKDKKRFEKANQDSGPGLSLEEFIAFEHPEEVDYMTEFVIQEALEEHDKNGDGFVSLEEFLGDYRWDPTANEDPEWILVEKDRFVNDYDKDNDGRLDP
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Predicted Molecular Mass
27.2 kDa
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Molecular Weight
Approximately 38 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (261 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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