RECQ4 Protein, Human (sf9, His, StrepⅡ)
RECQ4 Protein, Human (sf9, His, Strep) is the recombinant human-derived RECQ4, expressed by sf9 insect cells , with Strep, His labeled tag. ,
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
RECQ4 Protein, Human (sf9, His, Strep) is the recombinant human-derived RECQ4, expressed by sf9 insect cells , with Strep, His labeled tag. ,
Background
RECQ4 is an ATP-dependent DNA helicase that unwinds dsDNA with a 3'-overhang in a 3'-5' direction but cannot unwind more than 18 bp of dsDNA. It may modulate chromosome segregation. The N-terminal domain (residues 1-54) binds Y-shaped DNA more efficiently than ssDNA or dsDNA, while the core helicase domain specifically binds ssDNA. by similar: Unwinding activity characteristics (3'-5' directionality and length limitation) and functional differences between domains (N-terminal vs. core helicase domain).
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-StrepⅡ;His
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Accession
O94761 (A427-P1116)
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Gene ID9401
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Protein Length
Partial
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Synonyms
RECQL4; RecQ Protein-Like 4; RecQ Like Helicase 4; DNA Helicase, RecQ-Like, Type 4; RecQ4; DNA Helicase, RecQ-Like Type 4; ATP-Dependent DNA Helicase Q4; DNA 3'-5' Helicase; DNA 3'-5' Helicase RecQ4; RECQ4; RecQ Helicase-Like 4; RTS
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AA Sequence
AVGPEPLVPSPQPVPEVPSLDPTVLPLYSLGPSGQLAETPAEVFQALEQLGHQAFRPGQERAVMRILSGISTLLVLPTGAGKSLCYQLPALLYSRRSPCLTLVVSPLLSLMDDQVSGLPPCLKAACIHSGMTRKQRESVLQKIRAAQVHVLMLTPEALVGAGGLPPAAQLPPVAFACIDEAHCLSQWSHNFRPCYLRVCKVLRERMGVHCFLGLTATATRRTASDVAQHLAVAEEPDLHGPAPVPTNLHLSVSMDRDTDQALLTLLQGKRFQNLDSIIIYCNRREDTERIAALLRTCLHAAWVPGSGGRAPKTTAEAYHAGMCSRERRRVQRAFMQGQLRVVVATVAFGMGLDRPDVRAVLHLGLPPSFESYVQAVGRAGRDGQPAHCHLFLQPQGEDLRELRRHVHADSTDFLAVKRLVQRVFPACTCTCTRPPSEQEGAVGGERPVPKYPPQEAEQLSHQAAPGPRRVCMGHERALPIQLTVQALDMPEEAIETLLCYLELHPHHWLELLATTYTHCRLNCPGGPAQLQALAHRCPPLAVCLAQQLPEDPGQGSSSVEFDMVKLVDSMGWELASVRRALCQLQWDHEPRTGVRRGTGVLVEFSELAFHLRSPGDLTAEEKDQICDFLYGRVQARERQALARLRRTFQAFHSVAFPSCGPCLEQQDEERSTRLKDLLGRYFEEEEGQEP
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Predicted Molecular Mass
79 kDa
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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