ROR2 Protein, Human (HEK293, His)
Based on 1 Customer Validation
The ROR2 protein is a tyrosine protein kinase receptor that plays a critical role in early chondrocyte formation and is essential for cartilage and growth plate development. Phosphorylated YWHAB induces osteogenesis and bone formation. ROR2 Protein, Human (HEK293, His) is the recombinant human-derived ROR2 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The ROR2 protein is a tyrosine protein kinase receptor that plays a critical role in early chondrocyte formation and is essential for cartilage and growth plate development. Phosphorylated YWHAB induces osteogenesis and bone formation. ROR2 Protein, Human (HEK293, His) is the recombinant human-derived ROR2 protein, expressed by HEK293 , with C-6*His labeled tag.
Background
ROR2 protein, a tyrosine-protein kinase receptor, emerges as a key player in the early stages of chondrocyte formation and is deemed essential for cartilage and growth plate development. Its phosphorylation of YWHAB not only induces osteogenesis but also promotes bone formation. Despite exhibiting minimal tyrosine kinase activity in vitro, ROR2's intricate role extends to potentially acting as a receptor for the Wnt ligand WNT5A. This interaction may lead to the intriguing outcome of inhibiting WNT3A-mediated signaling. The multifaceted functions of ROR2 highlight its significance in orchestrating complex processes such as chondrogenesis, osteogenesis, and the intricate regulation of Wnt signaling pathways.
Verified Bioactivity
1.This product does not contain protein kinase domain.
2.Measured in a cell inhibition assay by using A375 cells. The ED50 this effect is 1.493 μg/mL, corresponding to a specific activity is 669.7924 units/mg.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
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Bioactivity - Cell-Based Assay
Bioactivity - Cell-Based Assay
Technical Parameters
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Species Human
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Source HEK293
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Tag C-His
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Accession
Q01974 (E34-G403)
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Molecular Construction
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N-term
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ROR2 (E34-G403)
Accession # Q01974 -
6*His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
ROR2; Tyrosine-Protein Kinase Transmembrane Receptor ROR2; Prev. NTRKR2; Receptor Tyrosine Kinase-Like Orphan Receptor 2, Isoform CRA_b; Prev. BDB1; Neurotrophic Tyrosine Kinase, Receptor-Related 2; Prev. BDB; Neurotrophic Tyrosine Kinase Receptor-Related
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AA Sequence
EVEVLDPNDPLGPLDGQDGPIPTLKGYFLNFLEPVNNITIVQGQTAILHCKVAGNPPPNVRWLKNDAPVVQEPRRIIIRKTEYGSRLRIQDLDTTDTGYYQCVATNGMKTITATGVLFVRLGPTHSPNHNFQDDYHEDGFCQPYRGIACARFIGNRTIYVDSLQMQGEIENRITAAFTMIGTSTHLSDQCSQFAIPSFCHFVFPLCDARSRTPKPRELCRDECEVLESDLCRQEYTIARSNPLILMRLQLPKCEALPMPESPDAANCMRIGIPAERLGRYHQCYNGSGMDYRGTASTTKSGHQCQPWALQHPHSHHLSSTDFPELGGGHAYCRNPGGQMEGPWCFTQNKNVRMELCDVPSCSPRDSSKMG
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Molecular Weight
Approximately 50-60 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS or 50 mM Tris-HCl, 300 mM NaCl, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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