ROR2 Protein, Mouse (HEK293, Fc)

The ROR2 protein is a tyrosine protein kinase receptor that plays a critical role in early chondrocyte formation and is essential for cartilage and growth plate development. Despite minimal tyrosine kinase activity, ROR2 phosphorylates YWHAB, which is associated with osteogenic induction and bone formation. ROR2 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived ROR2 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of ROR2 Protein, Mouse (HEK293, Fc) is 370 a.a., with molecular weight of 70-100 kDa.

ROR2 protein, a tyrosine-protein kinase receptor, emerges as a key player in the early stages of chondrocyte formation and is indispensable for cartilage and growth plate development. This receptor demonstrates the capability to phosphorylate YWHAB, an event associated with the induction of osteogenesis and bone formation. Intriguingly, although it exhibits minimal tyrosine kinase activity in vitro, ROR2 is proposed to act as a receptor for the WNT ligand WNT5A, potentially exerting inhibitory effects on WNT3A-mediated signaling. The nuanced role of ROR2 underscores its involvement in intricate cellular processes during skeletal development.

This product does not contain protein kinase domain.

Mouse

HEK293

C-hFc

Q9Z138-1 (E34-G403)

26564  [NCBI]

Extracellular Domain

EVEDSEAIDTLGQPDGPDSPLPTLKGYFLNFLEPVNNITIVQGQTAILHCKVAGNPPPNVRWLKNDAPVVQEPRRVIIRKTEYGSRLRIQDLDTTDTGYYQCVATNGLKTITATGVLYVRLGPTHSPNHNFQDDDQEDGFCQPYRGIACARFIGNRTIYVDSLQMQGEIENRITAAFTMIGTSTQLSDQCSQFAIPSFCHFVFPLCDARSRAPKPRELCRDECEVLENDLCRQEYTIARSNPLILMRLQLPKCEALPMPESPDAANCMRIGIPAERLGRYHQCYNGSGADYRGMASTTKSGHQCQPWALQHPHSHRLSSTEFPELGGGHAYCRNPGGQVEGPWCFTQNKNVRVELCDVPPCSPRDGSKMG

Approximately 70-100 kDa, based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized a 0.22 μm filtered solution of 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with 10% trehalose as protectant..
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.