RVFV-L Protein (His)
RVFV-L Protein (His) is the recombinant Virus-derived RVFV-L protein, expressed by E. coli, with C-His labeled tag.
- Species: Virus
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
RVFV-L Protein (His) is the recombinant Virus-derived RVFV-L protein, expressed by E. coli, with C-His labeled tag.
Background
RVFV-L is an RNA-dependent RNA polymerase responsible for replicating and transcribing the viral RNA genome using antigenomic RNA as an intermediate. During transcription, it synthesizes subgenomic RNAs and ensures their capping via a cap-snatching mechanism, which involves endonuclease activity cleaving host capped pre-mRNAs. These short capped RNAs are then used as primers for viral transcription. The 3'-ends of subgenomic mRNA molecules are not polyadenylated. During replication, the polymerase binds the 5' and 3' vRNA extremities at distinct sites, triggering significant conformational changes in both the polymerase and vRNA to initiate active RNA synthesis. Since the same enzyme is used for both transcription and replication, these mechanisms must be tightly coordinated.
Technical Parameters
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Species Virus
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Source E. coli
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Tag C-His
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Accession
AEB20483.1 (M1-F250)
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Gene ID/
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Molecular Construction
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N-term
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RVFV-L (M1-F250)
Accession # AEB20483.1 -
His
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C-term
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Protein Length
Partial
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Synonyms
L; Transcriptase; Replicase; EC:2.7.7.48; Large structural protein; RNA-directed RNA polymerase L
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AA Sequence
MDSILSKQLVDKTGFVRVPIKHYDCTMLTLALPTFDVSKMVDRITIDFNLDDIQGASEIGSTLLPSMSIDVEDMANFVHDFTFGHLADKTDRLLMREFPMMNDGFDHLSPDMIIKTTSGMYNIVEFTTFRGDERGAFQAAMTKLAKYEVPCENRSQGRTVVLYVVSAYRHGVWSNLELEDSEAEEMVYRYRLALSVMDELRTLFPELSSTDEELGKTERELLAMVSSIQINWSVTESVFPPFSREMFDKF
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Predicted Molecular Mass
29.4 kDa
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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