SENP5 Protein, Human (sf9, GST)

The SENP5 protein is critical in the SUMO pathway and promotes the regulation of SUMOylation through two key functions. First, it processes full-length SUMO3 into its mature form, facilitating the maturation of this ubiquitin-like modifier. SENP5 Protein, Human (sf9, GST) is the recombinant human-derived SENP5 protein, expressed by sf9 , with N-GST labeled tag.

SENP5 protein plays a pivotal role in the SUMO pathway, executing two crucial functions necessary for the regulation of SUMOylation dynamics. Firstly, it facilitates the processing of full-length SUMO3 to its mature form, contributing to the maturation of this small ubiquitin-like modifier. Additionally, SENP5 is actively involved in the deconjugation process, targeting SUMO2 and SUMO3 for removal from modified proteins. Although it exhibits weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates, SENP5's primary impact is on SUMO3-related processes. Its indispensable role in cell division further underscores its significance in fundamental cellular functions. Notably, SENP5 interacts with CCAR2, indicating potential regulatory associations in cellular processes influenced by SUMOylation.

SENP5 exhibits linear reaction kinetics under the condition of substrate SUMO2-AMC, with a concentration of 0.1 nM and a reaction time of 30 min, and the signal-to-noise ratio (S/N) is ≥5.

Human

Sf9 insect cells

N-GST

Q96HI0 (D536-D755)

205564

Partial

Approximately 50 kDa, based on SDS-PAGE under reducing conditions.

≥ 85%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.