Copy Link Print Information

Serpin F1 Protein, Mouse (375a.a)

Customer Review

Based on 1 Customer Validation

Serpin F1 is a neurotrophin that significantly induces extensive neuronal differentiation of retinoblastoma cells and exhibits potent anti-angiogenic effects.Its unique conformation lacks the S (stress) to R (relaxation) transition typical of active serpins, resulting in a lack of serpin activity.Serpin F1 Protein, Mouse is the recombinant mouse-derived Serpin F1 protein, expressed by E.coli , with tag free.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for Serpin F1 Protein, Mouse (375a.a)
  • Experimental Validation Results for Serpin F1 Protein, Mouse (375a.a)
  • Serpin F1 Protein, Mouse (375a.a) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

Serpin F1 is a neurotrophin that significantly induces extensive neuronal differentiation of retinoblastoma cells and exhibits potent anti-angiogenic effects.Its unique conformation lacks the S (stress) to R (relaxation) transition typical of active serpins, resulting in a lack of serpin activity.Serpin F1 Protein, Mouse is the recombinant mouse-derived Serpin F1 protein, expressed by E.coli , with tag free.

Background

Serpin F1, a neurotrophic protein, emerges as a key inducer of extensive neuronal differentiation in retinoblastoma cells. Notably, it exerts a potent inhibitory effect on angiogenesis. Distinguished by its unique conformational characteristics, Serpin F1 does not undergo the S (stressed) to R (relaxed) transition typical of active serpins, resulting in the absence of serine protease inhibitory activity. Furthermore, its interaction with PNPLA2 serves to stimulate the phospholipase A2 activity of PNPLA2, underscoring the multifaceted roles of Serpin F1 in cellular processes.

Verified Bioactivity

Measured by its ability to enhance the adhesion of Saos-2 human osteosarcoma cells to bovine Collagen I coated plate. The ED50 for this effect is 0.4644 μg/mL, corresponding to a specific activity is 2.153×103 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to enhance the adhesion of Saos-2 human osteosarcoma cells to bovine Collagen I coated plate. The ED50 for this effect is 0.4644 μg/mL, corresponding to a specific activity is 2.153×103 units/mg.

Technical Parameters

  • Species Mouse
  • Source E. coli
  • Tag Tag Free
  • Accession

    P97298 (D43-T417)

  • Gene ID
    20317  [NCBI]
  • Molecular Construction
    • N-term
    • Serpin F1 (D43-T417)
      Accession # P97298
    • C-term
  • Protein Length

    Partial

  • Synonyms

    SERPINF1; Testis Tissue Sperm-Binding Protein Li 70n; Prev. PEDF; Serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1; EPC-1; Proliferation-Inducing Protein 35; Pigment Epithelium-Derived Factor; Serpin F

  • AA Sequence

    DPFFKVPVNKLAAAVSNFGYDLYRLRSSASPTGNVLLSPLSVATALSALSLGAEHRTESVIHRALYYDLITNPDIHSTYKELLASVTAPEKNLKSASRIVFERKLRVKSSFVAPLEKSYGTRPRILTGNPRVDLQEINNWVQAQMKGKIARSTREMPSALSILLLGVAYFKGQWVTKFDSRKTTLQDFHLDEDRTVRVPMMSDPKAILRYGLDSDLNCKIAQLPLTGSMSIIFFLPLTVTQNLTMIEESLTSEFIHDIDRELKTIQAVLTVPKLKLSFEGELTKSLQDMKLQSLFESPDFSKITGKPVKLTQVEHRAAFEWNEEGAGSSPSPGLQPVRLTFPLDYHLNQPFLFVLRDTDTGALLFIGRILDPSST

  • Predicted Molecular Mass

    42 kDa

  • Molecular Weight

    Approximately 40 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to enhance the adhesion of Saos-2 human osteosarcoma cells to bovine Collagen I coated plate. The ED50 for this effect is 0.4644 μg/mL, corresponding to a specific activity is 2.153×103 units/mg.

  • Serpin F1 Protein, Mouse (375a.a) Product Image
    1/16
  • Experimental Validation Results for Serpin F1 Protein, Mouse (375a.a)
  • Experimental Validation Results for Serpin F1 Protein, Mouse (375a.a)
  • Serpin F1 Protein, Mouse (375a.a) Product Image