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sFRP-3 Protein, Mouse (HEK293, His)

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Based on 1 Customer Validation

The sFRP-3 protein is a soluble Frizzled-related protein that modulates Wnt signaling by directly interacting with Wnt, thereby regulating cell growth and differentiation. Known as FRZB, it acts as a Wnt8 signaling antagonist with crucial effects on limb skeletogenesis. sFRP-3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived sFRP-3 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for sFRP-3 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for sFRP-3 Protein, Mouse (HEK293, His)
  • sFRP-3 Protein, Mouse (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

The sFRP-3 protein is a soluble Frizzled-related protein that modulates Wnt signaling by directly interacting with Wnt, thereby regulating cell growth and differentiation. Known as FRZB, it acts as a Wnt8 signaling antagonist with crucial effects on limb skeletogenesis. sFRP-3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived sFRP-3 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

sFRP-3, a soluble frizzled-related protein, operates as a modulator of Wnt signaling through its direct interaction with Wnts, contributing to the regulation of cell growth and differentiation in specific cell types. Notably, SFRP-3, also known as FRZB, plays a key role in limb skeletogenesis, functioning as an antagonist of Wnt8 signaling. Its regulatory influence extends to chondrocyte maturation and long bone development. In addition to its participation in Wnt signaling modulation, SFRP-3 interacts with MYOC, showcasing its engagement in molecular interactions associated with cellular processes. These diverse functions underscore the multifaceted regulatory role of sFRP-3 in Wnt-mediated pathways and skeletogenesis.

Verified Bioactivity

Measured by its ability to inhibit proliferation in MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 5.797 µg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to inhibit proliferation of MC3T3-E1. The ED50 for this effect is 5.797 μg/mL, corresponding to a specific activity is 172.503 units/mg.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-6*His
  • Accession

    P97401 (A33-S323)

  • Gene ID
    20378
  • Molecular Construction
    • N-term
    • sFRP-3 (A33-S323)
      Accession # P97401
    • 6*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    FRZB; FZRB; Frizzled Related Protein; HFIZ; Secreted Frizzled-Related Protein 3; Frezzled; FRZB1; SFRP-3; SFRP3; FrzB-1; FRE; Uncharacterized Protein FRZB; Frizzled-Related Protein 1; Frizzled Homolog-Related; FRZB-PEN; Frizzled-Related Protein; FRZB-1; F

  • AA Sequence

    AACEPVRIPLCKSLPWNMTKMPNHLHHSTQANAILAMEQFEGLLGTHCSPDLLFFLCAMYAPICTIDFQHEPIKPCKSVCERARQGCEPILIKYRHSWPESLACDELPVYDRGVCISPEAIVTADGADFPMDSSTGHCRGASSERCKCKPVRATQKTYFRNNYNYVIRAKVKEVKMKCHDVTAVVEVKEILKASLVNIPRDTVNLYTTSGCLCPPLTVNEEYVIMGYEDEERSRLLLVEGSIAEKWKDRLGKKVKRWDMKLRHLGLGKTDASDSTQNQKSGRNSNPRPARS

  • Molecular Weight

    Approximately 37 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to inhibit proliferation of MC3T3-E1. The ED50 for this effect is 5.797 μg/mL, corresponding to a specific activity is 172.503 units/mg.

  • sFRP-3 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for sFRP-3 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for sFRP-3 Protein, Mouse (HEK293, His)
  • sFRP-3 Protein, Mouse (HEK293, His) Product Image