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SGSH Protein, Human (HEK293, His)

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SGSH (N-sulfoglucosamine sulfohydrolase) plays a key role in catalyzing specific steps in the lysosomal degradation of heparan sulfate. Through its enzymatic activity, SGSH contributes to the breakdown of heparan sulfate molecules, an important component of the extracellular matrix and cell surfaces. SGSH Protein, Human (HEK293, His) is the recombinant human-derived SGSH protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

SGSH (N-sulfoglucosamine sulfohydrolase) plays a key role in catalyzing specific steps in the lysosomal degradation of heparan sulfate. Through its enzymatic activity, SGSH contributes to the breakdown of heparan sulfate molecules, an important component of the extracellular matrix and cell surfaces. SGSH Protein, Human (HEK293, His) is the recombinant human-derived SGSH protein, expressed by HEK293 , with C-6*His labeled tag.

Background

SGSH, or N-sulfoglucosamine sulfohydrolase, plays a critical role in catalyzing a specific step in the degradation process of lysosomal heparan sulfate. Through its enzymatic activity, SGSH contributes to the breakdown of heparan sulfate molecules, which are essential components of the extracellular matrix and cell surface. This degradation process is crucial for maintaining cellular homeostasis and proper functioning of various tissues and organs. Understanding the precise mechanisms and regulation of SGSH-mediated degradation of lysosomal heparan sulfate can provide insights into the pathogenesis and potential therapeutic targets for lysosomal storage disorders associated with heparan sulfate accumulation.

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    P51688 (R21-L502)

  • Gene ID
    6448  [NCBI]
  • Molecular Construction
    • N-term
    • SGSH (R21-L502)
      Accession # P51688
    • 6*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    SGSH; Sulfoglucosamine Sulfamidase; N-Sulfoglucosamine Sulfohydrolase; Sulphamidase; HSS; N-Sulphoglucosamine Sulphohydrolase; MPS3A; Heparan Sulfate Sulfatase; SFMD; Sulfamidase; Mucopolysaccharidosis Type IIIA

  • AA Sequence

    RPRNALLLLADDGGFESGAYNNSAIATPHLDALARRSLLFRNAFTSVSSCSPSRASLLTGLPQHQNGMYGLHQDVHHFNSFDKVRSLPLLLSQAGVRTGIIGKKHVGPETVYPFDFAYTEENGSVLQVGRNITRIKLLVRKFLQTQDDRPFFLYVAFHDPHRCGHSQPQYGTFCEKFGNGESGMGRIPDWTPQAYDPLDVLVPYFVPNTPAARADLAAQYTTVGRMDQGVGLVLQELRDAGVLNDTLVIFTSDNGIPFPSGRTNLYWPGTAEPLLVSSPEHPKRWGQVSEAYVSLLDLTPTILDWFSIPYPSYAIFGSKTIHLTGRSLLPALEAEPLWATVFGSQSHHEVTMSYPMRSVQHRHFRLVHNLNFKMPFPIDQDFYVSPTFQDLLNRTTAGQPTGWYKDLRHYYYRARWELYDRSRDPHETQNLATDPRFAQLLEMLRDQLAKWQWETHDPWVCAPDGVLEEKLSPQCQPLHNEL

  • Predicted Molecular Mass

    55.7 kDa

  • Molecular Weight

    Approximately 60-75 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 1 mM CaCl2, 10% Glycerol, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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