SGSH Protein, Human (HEK293, His)

SGSH (N-sulfoglucosamine sulfohydrolase) plays a key role in catalyzing specific steps in the lysosomal degradation of heparan sulfate. Through its enzymatic activity, SGSH contributes to the breakdown of heparan sulfate molecules, an important component of the extracellular matrix and cell surfaces. SGSH Protein, Human (HEK293, His) is the recombinant human-derived SGSH protein, expressed by HEK293 , with C-6*His labeled tag.

SGSH, or N-sulfoglucosamine sulfohydrolase, plays a critical role in catalyzing a specific step in the degradation process of lysosomal heparan sulfate. Through its enzymatic activity, SGSH contributes to the breakdown of heparan sulfate molecules, which are essential components of the extracellular matrix and cell surface. This degradation process is crucial for maintaining cellular homeostasis and proper functioning of various tissues and organs. Understanding the precise mechanisms and regulation of SGSH-mediated degradation of lysosomal heparan sulfate can provide insights into the pathogenesis and potential therapeutic targets for lysosomal storage disorders associated with heparan sulfate accumulation.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

P51688 (R21-L502)

6448  [NCBI]

Full Length of Mature Protein

RPRNALLLLADDGGFESGAYNNSAIATPHLDALARRSLLFRNAFTSVSSCSPSRASLLTGLPQHQNGMYGLHQDVHHFNSFDKVRSLPLLLSQAGVRTGIIGKKHVGPETVYPFDFAYTEENGSVLQVGRNITRIKLLVRKFLQTQDDRPFFLYVAFHDPHRCGHSQPQYGTFCEKFGNGESGMGRIPDWTPQAYDPLDVLVPYFVPNTPAARADLAAQYTTVGRMDQGVGLVLQELRDAGVLNDTLVIFTSDNGIPFPSGRTNLYWPGTAEPLLVSSPEHPKRWGQVSEAYVSLLDLTPTILDWFSIPYPSYAIFGSKTIHLTGRSLLPALEAEPLWATVFGSQSHHEVTMSYPMRSVQHRHFRLVHNLNFKMPFPIDQDFYVSPTFQDLLNRTTAGQPTGWYKDLRHYYYRARWELYDRSRDPHETQNLATDPRFAQLLEMLRDQLAKWQWETHDPWVCAPDGVLEEKLSPQCQPLHNEL

Approximately 63 kDa,based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 1 mM CaCl₂, 10% Glycerol, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.