SGTA Protein, Human (His)
SGTA proteins play a key role in promoting the release of oligosaccharides from the asparagine-linked glycoprotein peptide backbone by specifically targeting the GlcNAc-Asn bond. This enzymatic activity is essential for glycoprotein processing and maturation, contributing to post-translational modifications and protein quality control. SGTA Protein, Human (His) is the recombinant human-derived SGTA protein, expressed by E. coli , with N-6*His labeled tag.
- Species: Human
- Source: E. coli
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
SGTA proteins play a key role in promoting the release of oligosaccharides from the asparagine-linked glycoprotein peptide backbone by specifically targeting the GlcNAc-Asn bond. This enzymatic activity is essential for glycoprotein processing and maturation, contributing to post-translational modifications and protein quality control. SGTA Protein, Human (His) is the recombinant human-derived SGTA protein, expressed by E. coli , with N-6*His labeled tag.
Background
AGA (Aspartylglucosaminidase) is a crucial enzyme that cleaves the GlcNAc-Asn bond, facilitating the release of oligosaccharides from the peptide backbone of asparagine-linked glycoproteins. This enzymatic activity is essential for the processing and maturation of glycoproteins, playing a key role in the intricate mechanisms of post-translational modification and protein quality control. By specifically targeting the GlcNAc-Asn linkage, AGA contributes to the generation of functionally mature glycoproteins, influencing various cellular processes. The precision of this cleavage highlights AGA's significance in maintaining the structural integrity and functionality of glycoproteins within the cellular milieu.
Technical Parameters
-
Species Human
-
Source E. coli
-
Tag N-6*His
-
Accession
O43765 (M1-E313)
-
Molecular Construction
-
N-term
-
6*His
-
SGTA (M1-E313)
Accession # O43765 -
C-term
-
-
Protein Length
Full Length
-
Synonyms
SGTA; HSGT; Prev. SGT; Small Glutamine-Rich Tetratricopeptide Repeat (TPR)-Containing, Alpha; SGT1; Small Glutamine Rich Tetratricopeptide Repeat Containing Alpha; UBP; Viral Protein U-Binding Protein; Small Glutamine-Rich Tetratricopeptide Repeat-Contain
-
AA Sequence
MDNKKRLAYAIIQFLHDQLRHGGLSSDAQESLEVAIQCLETAFGVTVEDSDLALPQTLPEIFEAAATGKEMPQDLRSPARTPPSEEDSAEAERLKTEGNEQMKVENFEAAVHFYGKAIELNPANAVYFCNRAAAYSKLGNYAGAVQDCERAICIDPAYSKAYGRMGLALSSLNKHVEAVAYYKKALELDPDNETYKSNLKIAELKLREAPSPTGGVGSFDIAGLLNNPGFMSMASNLMNNPQIQQLMSGMISGGNNPLGTPGTSPSQNDLASLIQAGQQFAQQMQQQNPELIEQLRSQIRSRTPSASNDDQQE
-
Predicted Molecular Mass
36.2 kDa
-
Molecular Weight
Approximately 35 kDa, based on SDS-PAGE under reducing conditions.
-
Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US;may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
