SIRP beta Protein, Mouse (HEK293, C-His)
Based on 1 Customer Validation
SIRP-beta (Sirpb, CD172b) is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins, Sirpb was found to interact with TYROBP/DAP12, a transmembrane adaptor protein bearing immunoreceptor tyrosine-based activation motifs, resulting in the tyrosine phosphorylation of DAP12 and the subsequent recruitment of Syk to the Sirpb-DAP12 complex. Sirpb is also implicated as a positive regulator of hematopoietic cells. SIRP beta Protein, Mouse (HEK293, His) is the recombinant mouse-derived SIRP beta protein, expressed by HEK293 , with C-His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
SIRP-beta (Sirpb, CD172b) is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins, Sirpb was found to interact with TYROBP/DAP12, a transmembrane adaptor protein bearing immunoreceptor tyrosine-based activation motifs, resulting in the tyrosine phosphorylation of DAP12 and the subsequent recruitment of Syk to the Sirpb-DAP12 complex. Sirpb is also implicated as a positive regulator of hematopoietic cells. SIRP beta Protein, Mouse (HEK293, His) is the recombinant mouse-derived SIRP beta protein, expressed by HEK293 , with C-His labeled tag.
Background
SIRP-beta (Sirpb, CD172b) is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily with three IgSF domains in its extracellular region. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Sirpb was found to interact with TYROBP/DAP12, a transmembrane adaptor protein bearing immunoreceptor tyrosine-based activation motifs. Ligation of Sirpb results in the tyrosine phosphorylation of DAP12 and the subsequent recruitment of Syk to the Sirpb-DAP12 complex in RBL-2H3 cell transfectants. It also stimulated serotonin release from these cells. Sirpb is therefore implicated as a positive regulator of hematopoietic cells[1][2].
Verified Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Mouse SIRP beta at 1μg/mL (100 μL/well) can bind Human SP-D (HY-P70986). The ED50 for this effect is 722.6 ng/mL.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-10*His
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Accession
BAD26610 (A27-K363)
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Gene ID/
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Molecular Construction
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N-term
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SIRP beta (A27-K363)
Accession # BAD26610 -
His
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C-term
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Protein Length
Partial
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Synonyms
Tyrosine-protein phosphatase non-receptor type substrate 1; Brain Ig-like molecule with tyrosine-based activation motifs; CD172 antigen-like family member A; Inhibitory receptor SHPS-1; Signal-regulatory protein alpha-1; Sirpb1a; SIRP-beta;
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AA Sequence
AMRELKVIQPVKSFFVGAGGSATLNCTVTSLLPVGPMRWYRGIGQSRLLIYSFTGEGFPRITNTSDTTKRNNMDFSIRISNVTPADSGTYYCVKFQRGPSDFYTEIQSGGGTELSVLAKPSSPMVSGPAARAVPQQTVTFTCRSHGFFPRNLTLKWFKNGDEISHLETSVEPEETSVSYRVSSTVQVVLEPRDVRSQIICEVDHVTLDRAPLRGIAHISEFIQVPPTLEIRQQPTMVWNVINVTCQIQKFYPPSFQLTWLENGNISRREVPFTLTVNKDGTYNWISCLLVNISALEENMVVTCQVEHDGQAEVIETHTVVVTEHQRVKELKTAGIAK
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Molecular Weight
Approximately 55-60 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.
<0.1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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