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  4. SLC12A2 Protein, Human (Sf9, His, Strep, FLAG)

SLC12A2 Protein, Human (Sf9, His, Strep, FLAG)

Cat. No.: HY-P702022
Handling Instructions

SLC12A2 Protein mediates electroneutral transport of chloride, potassium, and/or sodium ions across the membrane, crucial for regulating ionic balance and cell volume. SLC12A2 Protein, Human (Sf9, His, Strep, FLAG) is the recombinant human-derived SLC12A2 protein, expressed by Sf9 insect cells , with N-Strep, C-Flag, N-8*His labeled tag. The total length of SLC12A2 Protein, Human (Sf9, His, Strep, FLAG) is 1211 a.a., .

For research use only. We do not sell to patients.

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Description

SLC12A2 Protein mediates electroneutral transport of chloride, potassium, and/or sodium ions across the membrane, crucial for regulating ionic balance and cell volume. SLC12A2 Protein, Human (Sf9, His, Strep, FLAG) is the recombinant human-derived SLC12A2 protein, expressed by Sf9 insect cells , with N-Strep, C-Flag, N-8*His labeled tag. The total length of SLC12A2 Protein, Human (Sf9, His, Strep, FLAG) is 1211 a.a., .
Background

SLC12A2 protein functions as a cation-chloride cotransporter, facilitating the electroneutral transport of chloride, potassium, and/or sodium ions across cellular membranes. This transporter, as evidenced by various studies, holds a pivotal role in maintaining ionic balance and regulating cell volume. The intricate interplay between chloride, potassium, and sodium ions orchestrated by SLC12A2 underscores its significance in cellular homeostasis and ion transport processes, contributing to the overall functionality of the cell.
Species

Human
Source

Sf9 insect cells
Tag

N-Strep;C-Flag;N-8*His
Accession

P55011 (E2-S1212)
Gene ID

6558
Molecular Construction
N-term
8*His-Strep
SLC12A2 (E2-S1212)
Accession # P55011
Flag
C-term
Synonyms
SLC12A2; Solute carrier family 12 member 2; Basolateral Na-K-Cl symporter; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

SLC12A2 Protein, Human (Sf9, His, Strep, FLAG) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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