SLC16A3 Protein, Human (Cell-Free, His)
Based on 1 Customer Validation
SLC16A3 Protein, Human (Cell-Free, His) is the recombinant human-derived SLC16A3 protein, expressed by E. coli cell-free system, with N-10*His tag.
- Species: Human
- Source: E. coli Cell-free
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Biological Activity
Description
SLC16A3 Protein, Human (Cell-Free, His) is the recombinant human-derived SLC16A3 protein, expressed by E. coli cell-free system, with N-10*His tag.
Background
SLC16A3 is a proton-dependent monocarboxylate transporter that facilitates the transport of monocarboxylates such as L-lactate and pyruvate. It plays a predominant role in L-lactate efflux from highly glycolytic cells (By similar).
Technical Parameters
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Species Human
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Source E. coli Cell-free
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Tag N-10*His
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Accession
O15427 (M1-V465)
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Gene ID9123
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Molecular Construction
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N-term
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10*His
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SLC16A3 (M1-V465)
Accession # O15427 -
C-term
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Protein Length
Full Length
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Synonyms
SLC16A3; Solute Carrier Family 16 (Monocarboxylate Transporter), Member 3; Solute Carrier Family 16 Member 3; MCT 4; MCT3; Monocarboxylate Transporter 3; MCT4; MCT 3; Monocarboxylate Transporter 4; MCT-3; Solute Carrier Family 16, Member 3 (Monocarboxylic
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AA Sequence
MGGAVVDEGPTGVKAPDGGWGWAVLFGCFVITGFSYAFPKAVSVFFKELIQEFGIGYSDTAWISSILLAMLYGTGPLCSVCVNRFGCRPVMLVGGLFASLGMVAASFCRSIIQVYLTTGVITGLGLALNFQPSLIMLNRYFSKRRPMANGLAAAGSPVFLCALSPLGQLLQDRYGWRGGFLILGGLLLNCCVCAALMRPLVVTAQPGSGPPRPSRRLLDLSVFRDRGFVLYAVAASVMVLGLFVPPVFVVSYAKDLGVPDTKAAFLLTILGFIDIFARPAAGFVAGLGKVRPYSVYLFSFSMFFNGLADLAGSTAGDYGGLVVFCIFFGISYGMVGALQFEVLMAIVGTHKFSSAIGLVLLMEAVAVLVGPPSGGKLLDATHVYMYVFILAGAEVLTSSLILLLGNFFCIRKKPKEPQPEVAAAEEEKLHKPPADSGVDLREVEHFLKAEPEKNGEVVHTPETSV
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Predicted Molecular Mass
50.1 kDa
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Molecular Weight
Approximately 45-50 kDa (Monomer) & 120 kDa (Dimer), based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Structure/Form
Monomer
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, 0.05% Brij-78, 6% Trehalose, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)