SMOC1 Protein, Human (HEK293, His)

SMOC1 Protein is a matricellular protein that interferes with growth factor receptor signalling. SMOC1 Protein plays essential roles in both eye and limb development and is a regulator of osteoblast differentiation. SMOC1 Protein, Human (HEK293, His) is the recombinant human-derived SMOC1 protein, expressed by HEK293 , with C-8*His labeled tag.

SMOC1 Protein is a matricellular protein that interferes with growth factor receptor signalling. SMOC1 Protein plays essential roles in both eye and limb development and is a regulator of osteoblast differentiation. SMOC1 encodes a multi-domain secreted protein that may have a critical role in ocular and limb development. Mutations in this gene are associated with microphthalmia and limb anomalies^[1][2].

Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells. When 5 x 10⁴ cells/well are added to rhSMOC-1 coated plates, cell adhesion is enhanced in a dose dependent manner after 75 minutes at 37°C. The ED₅₀ for this effect is 0.5-1.5 μg/mL.

• 
  Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells. When 5 x 10⁴ cells/well are added to rhSMOC-1 coated plates, cell adhesion is enhanced in a dose dependent manner after 75 minutes at 37°C. The ED₅₀ for this effect is 0.5833 μg/mL, corresponding to a specific activity is 1714.384 units/mg.

Human

HEK293

C-8*His

Q9H4F8-2/NP_001030024.1 (H27-V435)

64093  [NCBI]

Partial

HRTTGPRFLISDRDPQCNLHCSRTQPKPICASDGRSYESMCEYQRAKCRDPTLGVVHRGRCKDAGQSKCRLERAQALEQAKKPQEAVFVPECGEDGSFTQVQCHTYTGYCWCVTPDGKPISGSSVQNKTPVCSGSVTDKPLSQGNSGRKDDGSKPTPTMETQPVFDGDEITAPTLWIKHLVIKDSKLNNTNIRNSEKVYSCDQERQSALEEAQQNPREGIVIPECAPGGLYKPVQCHQSTGYCWCVLVDTGRPLPGTSTRYVMPSCESDARAKTTEADDPFKDRELPGCPEGKKMEFITSLLDALTTDMVQAINSAAPTGGGRFSEPDPSHTLEERVVHWYFSQLDSNSSNDINKREMKPFKRYVKKKAKPKKCARRFTDYCDLNKDKVISLPELKGCLGVSKEVGRLV

55-70 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.