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SMOC1 Protein, Human (HEK293, His)

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Based on 1 Customer Validation

SMOC1 Protein is a matricellular protein that interferes with growth factor receptor signalling. SMOC1 Protein plays essential roles in both eye and limb development and is a regulator of osteoblast differentiation. SMOC1 Protein, Human (HEK293, His) is the recombinant human-derived SMOC1 protein, expressed by HEK293 , with C-8*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for SMOC1 Protein, Human (HEK293, His)
  • Experimental Validation Results for SMOC1 Protein, Human (HEK293, His)
  • SMOC1 Protein, Human (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

SMOC1 Protein is a matricellular protein that interferes with growth factor receptor signalling. SMOC1 Protein plays essential roles in both eye and limb development and is a regulator of osteoblast differentiation. SMOC1 Protein, Human (HEK293, His) is the recombinant human-derived SMOC1 protein, expressed by HEK293 , with C-8*His labeled tag.

Background

SMOC1 Protein is a matricellular protein that interferes with growth factor receptor signalling. SMOC1 Protein plays essential roles in both eye and limb development and is a regulator of osteoblast differentiation. SMOC1 encodes a multi-domain secreted protein that may have a critical role in ocular and limb development. Mutations in this gene are associated with microphthalmia and limb anomalies[1][2].

Verified Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells. When 5 x 104 cells/well are added to rhSMOC-1 coated plates, cell adhesion is enhanced in a dose dependent manner after 75 minutes at 37°C. The ED50 for this effect is 0.5-1.5 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells. When 5 x 104 cells/well are added to rhSMOC-1 coated plates, cell adhesion is enhanced in a dose dependent manner after 75 minutes at 37°C. The ED50 for this effect is 0.5833 μg/mL, corresponding to a specific activity is 1714.384 units/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-8*His
  • Accession

    Q9H4F8-2/NP_001030024.1 (H27-V435)

  • Gene ID
    64093  [NCBI]
  • Molecular Construction
    • N-term
    • SMOC1 (H27-V435)
      Accession # Q9H4F8-2/NP_001030024.1
    • His
    • C-term
  • Protein Length

    Full Length of SPARC-related modular calcium-binding protein 1 Chain

  • Synonyms

    SMOC1; Secreted Modular Calcium-Binding Protein 1; SPARC Related Modular Calcium Binding 1; SMOC-1; SPARC-Related Modular Calcium-Binding Protein 1; OAS

  • AA Sequence

    HRTTGPRFLISDRDPQCNLHCSRTQPKPICASDGRSYESMCEYQRAKCRDPTLGVVHRGRCKDAGQSKCRLERAQALEQAKKPQEAVFVPECGEDGSFTQVQCHTYTGYCWCVTPDGKPISGSSVQNKTPVCSGSVTDKPLSQGNSGRKDDGSKPTPTMETQPVFDGDEITAPTLWIKHLVIKDSKLNNTNIRNSEKVYSCDQERQSALEEAQQNPREGIVIPECAPGGLYKPVQCHQSTGYCWCVLVDTGRPLPGTSTRYVMPSCESDARAKTTEADDPFKDRELPGCPEGKKMEFITSLLDALTTDMVQAINSAAPTGGGRFSEPDPSHTLEERVVHWYFSQLDSNSSNDINKREMKPFKRYVKKKAKPKKCARRFTDYCDLNKDKVISLPELKGCLGVSKEVGRLV

  • Molecular Weight

    Approximately 55-60 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells. When 5 x 104 cells/well are added to rhSMOC-1 coated plates, cell adhesion is enhanced in a dose dependent manner after 75 minutes at 37°C. The ED50 for this effect is 0.5833 μg/mL, corresponding to a specific activity is 1714.384 units/mg.

  • SMOC1 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for SMOC1 Protein, Human (HEK293, His)
  • Experimental Validation Results for SMOC1 Protein, Human (HEK293, His)
  • SMOC1 Protein, Human (HEK293, His) Product Image