SPSB4 Protein, Human (His)
SPSB4 Protein, Human (His) is the recombinant human-derived SPSB4, expressed by E. coli , with His labeled tag. ,
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
SPSB4 Protein, Human (His) is the recombinant human-derived SPSB4, expressed by E. coli , with His labeled tag. ,
SPSB4 is the substrate recognition component of an SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex, mediating ubiquitination and subsequent proteasomal degradation of target proteins (by similar). It negatively regulates nitric oxide (NO) production and limits cellular toxicity in activated macrophages by mediating the ubiquitination and proteasomal degradation of NOS2 (by similar). SPSB4 acts as a bridge linking NOS2 with the ECS E3 ligase complex components ELOC and CUL5 (by similar). It diminishes EphB2-dependent cell repulsive responses by mediating the ubiquitination and degradation of EphB2/CTF2 (by similar), and regulates cellular clock function by mediating the ubiquitin/proteasome-dependent degradation of the circadian transcriptional repressor NR1D1.
Technical Parameters
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Species Human
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Source E. coli
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Tag His
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Accession
Q96A44 (R33-E233)
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Gene ID92369
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Protein Length
Partial
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Synonyms
SPSB4; SPRY Domain-Containing SOCS Box Protein 4; SplA/Ryanodine Receptor Domain And SOCS Box Containing 4; SSB4; SSB-4; SPRY Domain-Containing SOCS Box Protein SSB-4
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AA Sequence
RPARLDQLLDMPAAGLAVQLRHAWNPEDRSLNVFVKDDDRLTFHRHPVAQSTDGIRGKVGHARGLHAWQINWPARQRGTHAVVGVATARAPLHSVGYTALVGSDAESWGWDLGRSRLYHDGKNQPGVAYPAFLGPDEAFALPDSLLVVLDMDEGTLSFIVDGQYLGVAFRGLKGKKLYPVVSAVWGHCEVTMRYINGLDPE
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Predicted Molecular Mass
23.7 kDa
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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