SRP54 Protein, Human (sf9, His)

SRP54 is an important component of the signal recognition particle (SRP) complex that drives co-translational targeting of secreted proteins to the endoplasmic reticulum (ER). SRP54 cooperates with the SRP receptor (SR) component SRPRA to bind to signal sequences on emerging ribosomal proteins. SRP54 Protein, Human (sf9, His) is the recombinant human-derived SRP54 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of SRP54 Protein, Human (sf9, His) is 504 a.a., with molecular weight of ~58 kDa.

SRP54, an essential component of the signal recognition particle (SRP) complex, contributes to the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). Within the SRP complex, SRP54 associates with the SRP receptor (SR) component SRPRA to facilitate the targeting of secretory proteins to the ER membrane. Functioning at the forefront of this process, SRP54 binds to the signal sequence of presecretory proteins as they emerge from the ribosomes. Notably, SRP54 exhibits basal GTPase activity and collaborates in the reciprocal GTPase activation of the SR subunit SRPRA, forming a GTP-dependent complex crucial for SRP-mediated cotranslational protein translocation into the ER. The presence of SRP9/SRP14 and/or SRP19 is required for the stable interaction of SRP54 with RNA. Beyond its role in protein translocation, SRP54 plays a significant part in diverse cellular processes, including the regulation of granulocytic cell proliferation, neutrophil migration capacity, and the development of the exocrine pancreas.

Human

Sf9 insect cells

N-His

P61011 (M1-M504)

6729  [NCBI]

Approximately 58 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.