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TAGLN2 Protein, Human (HEK293, C-His)

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The human isoform of TAGLN2 protein is expressed in C-His-tagged HEK 293 cells and bears similarity to transgelin, an early marker of differentiated smooth muscle. Although its specific function is unknown, TAGLN2 is thought to be a tumor suppressor. TAGLN2 Protein,Human (HEK 293, C-His) is the recombinant human-derived TAGLN2 protein,Human(HEK 293, C-His), expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for TAGLN2 Protein, Human (HEK293, C-His)
  • TAGLN2 Protein, Human (HEK293, C-His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The human isoform of TAGLN2 protein is expressed in C-His-tagged HEK 293 cells and bears similarity to transgelin, an early marker of differentiated smooth muscle. Although its specific function is unknown, TAGLN2 is thought to be a tumor suppressor. TAGLN2 Protein,Human (HEK 293, C-His) is the recombinant human-derived TAGLN2 protein,Human(HEK 293, C-His), expressed by HEK293 , with C-6*His labeled tag.

Background

The TAGLN2 Protein, particularly in its human isoform expressed in HEK 293 cells with a C-His tag, shares similarity with transgelin, an early marker of differentiated smooth muscle. While the specific function of TAGLN2 has not been conclusively determined, it is proposed to function as a tumor suppressor. The gene exhibits multiple transcript variants that encode different isoforms, contributing to the complexity of its potential roles. This TAGLN2 protein demonstrates ubiquitous expression, with notable levels detected in the lung (RPKM 268.8), stomach (RPKM 231.0), and 25 other tissues. Its widespread expression pattern suggests involvement in various physiological contexts across multiple organs.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    P37802/NP_003555 (M1-L199)

  • Gene ID
    8407
  • Molecular Construction
    • N-term
    • TAGLN2 (M1-L199)
      Accession # NP_003555
    • 6*His
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    TAGLN2; Epididymis Tissue Protein Li 7e; Transgelin 2; Transgelin-2; SM22-Alpha Homolog; Epididymis Secretory Sperm Binding Protein; KIAA0120; Transgelin; HA1756

  • AA Sequence

    MANRGPAYGLSREVQQKIEKQYDADLEQILIQWITTQCRKDVGRPQPGRENFQNWLKDGTVLCELINALYPEGQAPVKKIQASTMAFKQMEQISQFLQAAERYGINTTDIFQTVDLWEGKNMACVQRTLMNLGGLAVARDDGLFSGDPNWFPKKSKENPRNFSDNQLQEGKNVIGLQMGTNRGASQAGMTGYGMPRQIL

  • Predicted Molecular Mass

    23.2 kDa

  • Molecular Weight

    Approximately 25-30 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • TAGLN2 Protein, Human (HEK293, C-His) Product Image
    1/16
  • Experimental Validation Results for TAGLN2 Protein, Human (HEK293, C-His)
  • TAGLN2 Protein, Human (HEK293, C-His) Product Image