TDT Protein, Bos taurus (His)

The TDT protein is a template-independent DNA polymerase that randomly adds deoxynucleoside 5'-triphosphates to the 3' end of the DNA initiator. Notably, its in vivo effects involve diversifying the immunoglobulin and T-cell receptor repertoire by adding nucleotides at the junction (N-region) of rearranged gene segments during B- and T-cell maturation. TDT Protein, Bos taurus (His) is the recombinant TDT protein, expressed by E. coli , with N-6*His labeled tag. TDT Protein, Bos taurus (His), has molecular weight of ~58.3 kDa.

The TDT protein, functioning as a template-independent DNA polymerase, catalyzes the stochastic addition of deoxynucleoside 5'-triphosphates to the 3'-end of a DNA initiator, a process documented in scientific literature. Notably, one of its in vivo roles involves adding nucleotides at the junction (N region) of rearranged immunoglobulin (Ig) heavy chain and T-cell receptor gene segments during the maturation phases of B- and T-cells. This underscores TDT's specific contribution to the diversification of immunoglobulin and T-cell receptor repertoires, elucidating its essential role in shaping the adaptive immune response during the maturation processes of both B- and T-cells.

Others

E. coli

N-6*His

P06526

281120  [NCBI]

MDPLCTASSGPRKKRPRQVGASMASPPHDIKFQNLVLFILEKKMGTTRRNFLMELARRKGFRVENELSDSVTHIVAENNSGSEVLEWLQVQNIRASSQLELLDVSWLIESMGAGKPVEITGKHQLVVRTDYSATPNPGFQKTPPLAVKKISQYACQRKTTLNNYNHIFTDAFEILAENSEFKENEVSYVTFMRAASVLKSLPFTIISMKDTEGIPCLGDKVKCIIEEIIEDGESSEVKAVLNDERYQSFKLFTSVFGVGLKTSEKWFRMGFRSLSKIMSDKTLKFTKMQKAGFLYYEDLVSCVTRAEAEAVGVLVKEAVWAFLPDAFVTMTGGFRRGKKIGHDVDFLITSPGSAEDEEQLLPKVINLWEKKGLLLYYDLVESTFEKFKLPSRQVDTLDHFQKCFLILKLHHQRVDSSKSNQQEGKTWKAIRVDLVMCPYENRAFALLGWTGSRQFERDIRRYATHERKMMLDNHALYDKTKRVFLKAESEEEIFAHLGLDYIEPWERNA

Approximately 58.3 kDa

Greater than 80% as determined by reducing SDS-PAGE.