TDT Protein, Bos taurus (His)
The TDT protein is a template-independent DNA polymerase that randomly adds deoxynucleoside 5'-triphosphates to the 3' end of the DNA initiator. Notably, its in vivo effects involve diversifying the immunoglobulin and T-cell receptor repertoire by adding nucleotides at the junction (N-region) of rearranged gene segments during B- and T-cell maturation. TDT Protein, Bos taurus (His) is the recombinant TDT protein, expressed by E. coli , with N-6*His labeled tag. TDT Protein, Bos taurus (His), has molecular weight of ~58.3 kDa.
- Species: Others
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The TDT protein is a template-independent DNA polymerase that randomly adds deoxynucleoside 5'-triphosphates to the 3' end of the DNA initiator. Notably, its in vivo effects involve diversifying the immunoglobulin and T-cell receptor repertoire by adding nucleotides at the junction (N-region) of rearranged gene segments during B- and T-cell maturation. TDT Protein, Bos taurus (His) is the recombinant TDT protein, expressed by E. coli , with N-6*His labeled tag. TDT Protein, Bos taurus (His), has molecular weight of ~58.3 kDa.
Background
The TDT protein, functioning as a template-independent DNA polymerase, catalyzes the stochastic addition of deoxynucleoside 5'-triphosphates to the 3'-end of a DNA initiator, a process documented in scientific literature. Notably, one of its in vivo roles involves adding nucleotides at the junction (N region) of rearranged immunoglobulin (Ig) heavy chain and T-cell receptor gene segments during the maturation phases of B- and T-cells. This underscores TDT's specific contribution to the diversification of immunoglobulin and T-cell receptor repertoires, elucidating its essential role in shaping the adaptive immune response during the maturation processes of both B- and T-cells.
Technical Parameters
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Species Others
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Source E. coli
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Tag N-6*His
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Accession
P06526 (M1-A509)
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Molecular Construction
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N-term
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6*His
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TDT (M1-A509)
Accession # P06526 -
C-term
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Protein Length
Full Length
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Synonyms
DNTT; Terminal Transferase; DNA Nucleotidylexotransferase; Nucleosidetriphosphate:DNA Deoxynucleotidylexotransferase; TDT; Terminal Deoxyribonucleotidyltransferase; Terminal Deoxynucleotidyltransferase; Deoxynucleotidyltransferase, Terminal; Terminal Addi
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AA Sequence
MDPLCTASSGPRKKRPRQVGASMASPPHDIKFQNLVLFILEKKMGTTRRNFLMELARRKGFRVENELSDSVTHIVAENNSGSEVLEWLQVQNIRASSQLELLDVSWLIESMGAGKPVEITGKHQLVVRTDYSATPNPGFQKTPPLAVKKISQYACQRKTTLNNYNHIFTDAFEILAENSEFKENEVSYVTFMRAASVLKSLPFTIISMKDTEGIPCLGDKVKCIIEEIIEDGESSEVKAVLNDERYQSFKLFTSVFGVGLKTSEKWFRMGFRSLSKIMSDKTLKFTKMQKAGFLYYEDLVSCVTRAEAEAVGVLVKEAVWAFLPDAFVTMTGGFRRGKKIGHDVDFLITSPGSAEDEEQLLPKVINLWEKKGLLLYYDLVESTFEKFKLPSRQVDTLDHFQKCFLILKLHHQRVDSSKSNQQEGKTWKAIRVDLVMCPYENRAFALLGWTGSRQFERDIRRYATHERKMMLDNHALYDKTKRVFLKAESEEEIFAHLGLDYIEPWERNA
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Molecular Weight
Approximately 58 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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