TDT Protein, Human (His)
Based on 1 Customer Validation
TDT protein, a template-independent DNA polymerase, non-specifically adds deoxynucleoside 5'-triphosphates to the 3'-end of a DNA initiator. It crucially contributes to in vivo processes, particularly adding nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during B- and T-cell maturation. TDT Protein, Human (His) is the recombinant human-derived TDT protein, expressed by E. coli , with N-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
TDT protein, a template-independent DNA polymerase, non-specifically adds deoxynucleoside 5'-triphosphates to the 3'-end of a DNA initiator. It crucially contributes to in vivo processes, particularly adding nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during B- and T-cell maturation. TDT Protein, Human (His) is the recombinant human-derived TDT protein, expressed by E. coli , with N-6*His labeled tag.
The TDT protein is a template-independent DNA polymerase that carries out the non-specific addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of its important roles in vivo is adding nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation process of B- and T-cells.
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His
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Accession
P04053 (M1-A509)
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Molecular Construction
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N-term
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6*His
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TDT (M1-A509)
Accession # P04053 -
C-term
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Protein Length
Full Length
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Synonyms
DNTT; Terminal Transferase; DNA Nucleotidylexotransferase; Nucleosidetriphosphate:DNA Deoxynucleotidylexotransferase; TDT; Terminal Deoxyribonucleotidyltransferase; Terminal Deoxynucleotidyltransferase; Deoxynucleotidyltransferase, Terminal; Terminal Addi
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AA Sequence
MDPPRASHLSPRKKRPRQTGALMASSPQDIKFQDLVVFILEKKMGTTRRAFLMELARRKGFRVENELSDSVTHIVAENNSGSDVLEWLQAQKVQVSSQPELLDVSWLIECIRAGKPVEMTGKHQLVVRRDYSDSTNPGPPKTPPIAVQKISQYACQRRTTLNNCNQIFTDAFDILAENCEFRENEDSCVTFMRAASVLKSLPFTIISMKDTEGIPCLGSKVKGIIEEIIEDGESSEVKAVLNDERYQSFKLFTSVFGVGLKTSEKWFRMGFRTLSKVRSDKSLKFTRMQKAGFLYYEDLVSCVTRAEAEAVSVLVKEAVWAFLPDAFVTMTGGFRRGKKMGHDVDFLITSPGSTEDEEQLLQKVMNLWEKKGLLLYYDLVESTFEKLRLPSRKVDALDHFQKCFLIFKLPRQRVDSDQSSWQEGKTWKAIRVDLVLCPYERRAFALLGWTGSRQFERDLRRYATHERKMILDNHALYDKTKRIFLKAESEEEIFAHLGLDYIEPWERNA
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Predicted Molecular Mass
60.7 kDa
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Molecular Weight
Approximately 62 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 0.1 M KH2PO4, 200 mM KCl, 1 mM 2-Mercaptoethanol, 50% glycerol, pH 7.2.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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