TDT Protein, Human (His)

TDT protein, a template-independent DNA polymerase, non-specifically adds deoxynucleoside 5'-triphosphates to the 3'-end of a DNA initiator. It crucially contributes to in vivo processes, particularly adding nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during B- and T-cell maturation. TDT Protein, Human (His) is the recombinant human-derived TDT protein, expressed by E. coli , with N-6*His labeled tag. The total length of TDT Protein, Human (His) is 509 a.a., with molecular weight of ~62.0 kDa.

The TDT protein is a template-independent DNA polymerase that carries out the non-specific addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of its important roles in vivo is adding nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation process of B- and T-cells.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

P04053 (M1-A509)

1791  [NCBI]

MDPPRASHLSPRKKRPRQTGALMASSPQDIKFQDLVVFILEKKMGTTRRAFLMELARRKGFRVENELSDSVTHIVAENNSGSDVLEWLQAQKVQVSSQPELLDVSWLIECIRAGKPVEMTGKHQLVVRRDYSDSTNPGPPKTPPIAVQKISQYACQRRTTLNNCNQIFTDAFDILAENCEFRENEDSCVTFMRAASVLKSLPFTIISMKDTEGIPCLGSKVKGIIEEIIEDGESSEVKAVLNDERYQSFKLFTSVFGVGLKTSEKWFRMGFRTLSKVRSDKSLKFTRMQKAGFLYYEDLVSCVTRAEAEAVSVLVKEAVWAFLPDAFVTMTGGFRRGKKMGHDVDFLITSPGSTEDEEQLLQKVMNLWEKKGLLLYYDLVESTFEKLRLPSRKVDALDHFQKCFLIFKLPRQRVDSDQSSWQEGKTWKAIRVDLVLCPYERRAFALLGWTGSRQFERDLRRYATHERKMILDNHALYDKTKRIFLKAESEEEIFAHLGLDYIEPWERNA

Approximately 62.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 0.1 M KH₂PO₄, 200 mM KCl, 1 mM 2-Mercaptoethanol, 50% Glycerol, pH 7.2.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.