1. Recombinant Proteins
  2. CD Antigens Receptor Proteins
  3. T Cell CD Proteins Monocyte CD Proteins Epithelial cell CD Proteins Pattern Recognition Receptors
  4. CD282/TLR2 Toll-like Receptor
  5. Toll Like Receptor 2
  6. TLR2 Protein, Human (sf9, His)

TLR2 Protein, Human (sf9, His)

Cat. No.: HY-P73591
COA Handling Instructions

TLR2 is a protein that plays a critical role in recognizing and responding to various bacterial and mycobacterial pathogens. TLR2 Protein, Human (sf9, His) is the recombinant human-derived TLR2 protein, expressed by Sf9 insect cells , with C-10*His labeled tag. The total length of TLR2 Protein, Human (sf9, His) is 567 a.a., with molecular weight of ~65.5 kDa.

For research use only. We do not sell to patients.

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Description

TLR2 is a protein that plays a critical role in recognizing and responding to various bacterial and mycobacterial pathogens. TLR2 Protein, Human (sf9, His) is the recombinant human-derived TLR2 protein, expressed by Sf9 insect cells , with C-10*His labeled tag. The total length of TLR2 Protein, Human (sf9, His) is 567 a.a., with molecular weight of ~65.5 kDa.

Background

TLR2 protein collaborates with LY96 to orchestrate the innate immune response against bacterial lipoproteins and various microbial cell wall components. Additionally, TLR2 forms functional complexes with either TLR1 or TLR6 to recognize bacterial lipopeptides. Through the signaling pathways involving MYD88 and TRAF6, TLR2 activation leads to NF-kappa-B activation, cytokine secretion, and the initiation of inflammatory responses. The receptor plays a pivotal role in recognizing specific ligands, such as mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2) and B.burgdorferi outer surface protein A lipoprotein (OspA-L), often in cooperation with TLR6. TLR2 engages in complex interactions with other receptors, including CD14 and CD36, to form activation clusters that trigger signaling events at the cell surface before being directed to the Golgi in a lipid-raft dependent pathway. Furthermore, TLR2 is crucial for the normal uptake of M.tuberculosis, and its interactions extend to various regulatory proteins such as MYD88, TICAM1, and TIRAP.

Species

Human

Source

Sf9 insect cells

Tag

C-10*His

Accession

O60603 (E21-R587)

Gene ID
Molecular Construction
N-term
TLR2 (E21-R587)
Accession # O60603
10*His
C-term
Synonyms
CD282; TIL4; TLR2; Toll-like receptor 2
AA Sequence

ESSNQASLSCDRNGICKGSSGSLNSIPSGLTEAVKSLDLSNNRITYISNSDLQRCVNLQALVLTSNGINTIEEDSFSSLGSLEHLDLSYNYLSNLSSSWFKPLSSLTFLNLLGNPYKTLGETSLFSHLTKLQILRVGNMDTFTKIQRKDFAGLTFLEELEIDASDLQSYEPKSLKSIQNVSHLILHMKQHILLLEIFVDVTSSVECLELRDTDLDTFHFSELSTGETNSLIKKFTFRNVKITDESLFQVMKLLNQISGLLELEFDDCTLNGVGNFRASDNDRVIDPGKVETLTIRRLHIPRFYLFYDLSTLYSLTERVKRITVENSKVFLVPCLLSQHLKSLEYLDLSENLMVEEYLKNSACEDAWPSLQTLILRQNHLASLEKTGETLLTLKNLTNIDISKNSFHSMPETCQWPEKMKYLNLSSTRIHSVTGCIPKTLEILDVSNNNLNLFSLNLPQLKELYISRNKLMTLPDASLLPMLLVLKISRNAITTFSKEQLDSFHTLKTLEAGGNNFICSCEFLSFTQEQQALAKVLIDWPANYLCDSPSHVRGQQVQDVRLSVSECHR

Molecular Weight

Approximately 65.5 kDa

Purity

Greater than 85% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.5, 10% Glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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TLR2 Protein, Human (sf9, His)
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HY-P73591
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