TLR3 Protein, Human (HEK293, His)
Based on 1 Customer Validation
The TLR3 protein is a key Toll-like receptor family member that plays an important role in the innate immune system's recognition of pathogen-associated molecular patterns, especially viral double-stranded RNA. Its research enhances understanding of immune responses and provides potential applications for vaccine development and antiviral treatments. TLR3 Protein, Human (HEK293, His) is the recombinant human-derived TLR3 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The TLR3 protein is a key Toll-like receptor family member that plays an important role in the innate immune system's recognition of pathogen-associated molecular patterns, especially viral double-stranded RNA. Its research enhances understanding of immune responses and provides potential applications for vaccine development and antiviral treatments. TLR3 Protein, Human (HEK293, His) is the recombinant human-derived TLR3 protein, expressed by HEK293 , with C-His labeled tag.
Background
The TLR3 Protein is a pivotal member of the Toll-like receptor family, signifying its essential role in the innate immune system's recognition of pathogen-associated molecular patterns. As part of this family, TLR3 likely shares conserved structural and functional features with related receptors, emphasizing its involvement in detecting viral double-stranded RNA. The classification within the Toll-like receptor family underscores its specific designation within the broader context of pattern recognition receptors, providing insights into its unique contributions to immune responses. The study of TLR3 contributes to our understanding of its role in host defense against viral infections, offering potential applications in vaccine development and antiviral therapies. Further exploration of TLR3's role holds promise for enhancing our knowledge of its contributions to both normal immune surveillance and pathological conditions.
Verified Bioactivity
1.Immobilized Human TLR3 at 0.5 μg/mL (100μL/Well) on the plate. Dose response curve for Anti-TLR3 Antibody with the EC50 < 13.6 ng/mL determined by ELISA.
2.Measured by its ability to inhibit poly I:C induced IL-8 secretion by HEK293 human embryonic kidney cells transfected with TLR3. The ED50 for this effect is 4.109 µg/mL, corresponding to a specific activity is 243.368 U/mg in the presence of 0.2 µg/mL of poly I:C.
3.Human TLR3 Protein immobilized on CM5 Chip can bind Anti-TLR3/CD283 Antibody (HY-P990519) with an affinity constant of 2.21 nM as determined in SPR assay.
4.Anti-TLR3/CD283 Antibody (HY-P990519) immobilized on CM5 Chip can bind Human TLR3 Protein with an affinity constant of 2.46 nM as determined in SPR assay.
MCE Validation Data
-
Purity - SDS-PAGE
Purity - SDS-PAGE
-
Bioactivity - Cell-Based Assay
Bioactivity - Cell-Based Assay
-
Bioactivity - SPR
Bioactivity - SPR
-
Bioactivity - SPR
Bioactivity - SPR
Technical Parameters
-
Species Human
-
Source HEK293
-
Tag C-His
-
Accession
Q6PCD4 (S23-E703)
-
Gene ID/
-
Molecular Construction
-
N-term
-
TLR3 (S23-E703)
Accession # Q6PCD4 -
His
-
C-term
-
-
Protein Length
Partial
-
Synonyms
TLR3; CD283 Antigen; Toll Like Receptor 3; TLR3 Protein; Toll-Like Receptor 3; IIAE2; CD283; IMD83; Toll-Like Receptor 3, Isoform CRA_b
-
AA Sequence
SSTTKCTVSHEVADCSHLKLTQVPDDLPTNITVLNLTHNQLRRLPAANFTRYSQLTSLDVGFNTISKLEPELCQKLPMLKVLNLQHNELSQLSDKTFAFCTNLTELHLMSNSIQKIKNNPFVKQKNLITLDLSHNGLSSTKLGTQVQLENLQELLLSNNKIQALKSEELDIFANSSLKKLELSSNQIKEFSPGCFHAIGRLFGLFLNNVQLGPSLTEKLCLELANTSIRNLSLSNSQLSTTSNTTFLGLKWTNLTMLDLSYNNLNVVGNDSFAWLPQLEYFFLEYNNIQHLFSHSLHGLFNVRYLNLKRSFTKQSISLASLPKIDDFSFQWLKCLEHLNMEDNDIPGIKSNMFTGLINLKYLSLSNSFTSLRTLTNETFVSLAHSPLHILNLTKNKISKIESDAFSWLGHLEVLDLGLNEIGQELTGQEWRGLENIFEIYLSYNKYLQLTRNSFALVPSLQRLMLRRVALKNVDSSPSPFQPLRNLTILDLSNNNIANINDDMLEGLEKLEILDLQHNNLARLWKHANPGGPIYFLKGLSHLHILNLESNGFDEIPVEVFKDLFELKIIDLGLNNLNTLPASVFNNQVSLKSLNLQKNLITSVEKKVFGPAFRNLTELDMRFNPFDCTCESIAWFVNWINETHTNIPELSSHYLCNTPPHYHGFPVRLFDTSSCKDSAPFE
-
Predicted Molecular Mass
78.7 kDa
-
Molecular Weight
Approximately 90-130 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
-
Glycosylation
Yes
-
Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm solution of PBS, pH 7.4
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
-
Data Sheet (240 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
