TMK Protein, Bacillus anthracis (His)
Based on 1 Customer Validation
TMK proteins play a central role by catalyzing the phosphorylation of deoxythymidine monophosphate (dTMP), a key step in the synthesis of deoxythymidine triphosphate (dTTP). This process converts dTMP into deoxythymidine diphosphate (dTDP), an important intermediate in dTTP biosynthesis. TMK Protein, Bacillus anthracis (His) is the recombinant TMK protein, expressed by E. coli , with N-6*His labeled tag.
- Species: Others
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
TMK proteins play a central role by catalyzing the phosphorylation of deoxythymidine monophosphate (dTMP), a key step in the synthesis of deoxythymidine triphosphate (dTTP). This process converts dTMP into deoxythymidine diphosphate (dTDP), an important intermediate in dTTP biosynthesis. TMK Protein, Bacillus anthracis (His) is the recombinant TMK protein, expressed by E. coli , with N-6*His labeled tag.
Background
The TMK Protein orchestrates a crucial role in catalyzing the phosphorylation of deoxythymidine monophosphate (dTMP), a pivotal step in the synthesis of deoxythymidine triphosphate (dTTP) within both the de novo and salvage pathways. This enzymatic process leads to the conversion of dTMP into deoxythymidine diphosphate (dTDP), serving as a vital intermediate in dTTP biosynthesis. The involvement of TMK Protein in facilitating this phosphorylation event underscores its central regulatory role in modulating the production of dTTP, a fundamental nucleotide essential for DNA synthesis. A comprehensive understanding of TMK Protein's engagement in these pathways enhances our insight into cellular nucleotide dynamics and, consequently, contributes to the maintenance of genomic stability.
Verified Bioactivity
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
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Species Others
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Source E. coli
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Tag N-6*His
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Accession
C3LJ02 (M1-L208)
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Gene ID45020070 [NCBI]
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Molecular Construction
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N-term
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6*His
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TMK (M1-L208)
Accession # C3LJ02 -
C-term
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Protein Length
Full Length
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Synonyms
Thymidylate kinase; EC:2.7.4.9; dTMP kinase; tmk; BAMEG_0039
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AA Sequence
MKGLFVTIEGPEGSGKTTLIQSLLPYFEQKEQKVMATREPGGIAISEDIRTILHKQEYTMMEARTEALLYAAARRQHLVEKVMPALNEDYLVLCDRFIDSSLAYQGYARGLGMDKVFEINRFATEDCMPSLTIYLDIEPEVGLARIAKDAGREVNRLDMEDISFHKRVREGYLQVVERFSDRIVLVNADQPMEKLIEEVIQVIEDKLL
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Predicted Molecular Mass
27.8 kDa
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Molecular Weight
Approximately 25 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 50 mM PBS, 300 mM NaCl, 5% trehalose, 5% mannitol and 0.01% Tween 80, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US;may vary elsewhere.
Documentation
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Data Sheet (236 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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