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  5. TMPRSS1/HPN Protein, Human (HEK293, His, solution)

TMPRSS1/HPN Protein, Human (HEK293, His, solution)

Cat. No.: HY-P77693Y
Handling Instructions Technical Support

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Biological Activity

1.Immobilized Human HPN, His Tag at 2 μg/ml (100 μl/Well) on the plate. Dose response curve for Anti-HPN Antibody, hFc Tag with the EC50 of 8.1 ng/ml determined by ELISA.
2.Measured by its ability to cleave tertbutoxycarbonyl-Gln-Arg-Arg-7-amino-4-methylcoumarin (Boc-QRR-AMC). The specific activity is 26434 pmol/min/μg.

Species

Human

Source

HEK293

Tag

C-10*His

Accession

P05981 (R45-L417)

Gene ID

3249

Molecular Construction
N-term
HPN (R45-L417)
Accession # P05981
10*His
C-term
Protein Length

Extracellular Domain

Synonyms
Hpxn; FLJ56652; Hemopexin; HPX; HX; Hepsin; HPN; TMPRSS1
AA Sequence

RSDQEPLYPVQVSSADARLMVFDKTEGTWRLLCSSRSNARVAGLSCEEMGFLRALTHSELDVRTAGANGTSGFFCVDEGRLPHTQRLLEVISVCDCPRGRFLAAICQDCGRRKLPVDRIVGGRDTSLGRWPWQVSLRYDGAHLCGGSLLSGDWVLTAAHCFPERNRVLSRWRVFAGAVAQASPHGLQLGVQAVVYHGGYLPFRDPNSEENSNDIALVHLSSPLPLTEYIQPVCLPAAGQALVDGKICTVTGWGNTQYYGQQAGVLQEARVPIISNDVCNGADFYGNQIKPKMFCAGYPEGGIDACQGDSGGPFVCEDSISRTPRWRLCGIVSWGTGCALAQKPGVYTKVSDFREWIFQAIKTHSEASGMVTQL

Predicted Molecular Mass
42 kDa.
Molecular Weight

Approximately 18-22 kDa and 30 kDa, based on Bis-Tris PAGE under reducing conditions

Glycosylation
Yes
Purity

≥ 95%, as determined by Bis-Tris PAGE.

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM NaAc,150 mM NaCl, pH 5.0.

Endotoxin Level

<0.5 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

TMPRSS1/HPN Protein, Human (HEK293, His, solution) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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TMPRSS1/HPN Protein, Human (HEK293, His, solution)
Cat. No.:
HY-P77693Y
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