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TOP2A Protein, Human

TOP2A Protein, Human is the recombinant human-derived TOP2A protein, expressed by E. coli, with tag free tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • TOP2A Protein, Human Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Product Properties
  • Documentation
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Biological Activity

Description

TOP2A Protein, Human is the recombinant human-derived TOP2A protein, expressed by E. coli, with tag free tag.

Background

TOP2A is a key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules. It generates a double-stranded break in one of the strands, passes the intact strand through the broken strand, and religates the broken strand. Additionally, TOP2A may play a role in regulating the period length of BMAL1 transcriptional oscillation (By similarity).

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag Tag Free
  • Accession

    P11388-1 (S1354-D1473)

  • Gene ID
    7153
  • Molecular Construction
    • N-term
    • TOP2A (S1354-S1473)
      Accession # P11388-1
    • C-term
  • Protein Length

    Partial

  • Synonyms

    TOP2A; DNA topoisomerase 2-alpha; TOP2; DNA topoisomerase II; alpha isozyme

  • AA Sequence

    SPPKTKTSPKLSNKELKPQKSVVSDLEADDVKGSVPLSSSPPATHFPDETEITNPVPKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDD

  • Predicted Molecular Mass

    13 kDa

  • Molecular Weight

    Approximately 20 kDa, based on SDS-PAGE under reducing conditions.

  • Structure/Form

    Monomer

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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  • TOP2A Protein, Human Product Image