TPM2 Protein, Human (His)
Based on 1 Customer Validation
The TPM2 protein binds actin filaments in muscle and nonmuscle cells and centrally regulates calcium-dependent striated muscle contraction in vertebrates, particularly in concert with the troponin complex. In smooth muscle cells, TPM2 helps regulate contraction by interacting with calcium-binding proteins. TPM2 Protein, Human (His) is the recombinant human-derived TPM2 protein, expressed by E. coli , with N-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The TPM2 protein binds actin filaments in muscle and nonmuscle cells and centrally regulates calcium-dependent striated muscle contraction in vertebrates, particularly in concert with the troponin complex. In smooth muscle cells, TPM2 helps regulate contraction by interacting with calcium-binding proteins. TPM2 Protein, Human (His) is the recombinant human-derived TPM2 protein, expressed by E. coli , with N-6*His labeled tag.
TPM2 protein exhibits specific binding to actin filaments in both muscle and non-muscle cells, playing a central role in the calcium-dependent regulation of vertebrate striated muscle contraction, particularly in association with the troponin complex. In smooth muscle cells, TPM2 contributes to the regulation of contraction through its interaction with caldesmon. Moreover, in non-muscle cells, TPM2 is implicated in the stabilization of actin filaments within the cytoskeleton, indicating its versatile functions across cellular contexts. The non-muscle isoform of TPM2 may additionally participate in agonist-mediated receptor internalization. Structurally, TPM2 forms homodimers and heterodimers, the latter composed of an alpha chain (TPM1, TPM3, or TPM4) and a beta chain (TPM2), highlighting its diverse molecular associations and underscoring its pivotal roles in both muscle contraction and cellular structural dynamics.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His
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Accession
P07951 (D14-L284)
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Molecular Construction
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N-term
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6*His
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TPM2 (D14-L284)
Accession # P07951 -
C-term
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Protein Length
Partial
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Synonyms
AMCD1; TMSA; TMSB; TPM2; TRK; Tropomyosin beta chain
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AA Sequence
DKENAIDRAEQAEADKKQAEDRCKQLEEEQQALQKKLKGTEDEVEKYSESVKEAQEKLEQAEKKATDAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRAMKDEEKMELQEMQLKEAKHIAEDSDRKYEEVARKLVILEGELERSEERAEVAESKCGDLEEELKIVTNNLKSLEAQADKYSTKEDKYEEEIKLLEEKLKEAETRAEFAERSVAKLEKTIDDLEDEVYAQKMKYKAISEELDNALNDITSL
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Predicted Molecular Mass
35.3 kDa
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Molecular Weight
Approximately 38 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
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≥ 90%, as determined by reducing SDS-PAGE.
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Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS , 6% trehalose, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (236 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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