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  3. TPSB2 Protein, Human (HEK293, His)

TPSB2 Protein, Human (HEK293, His) is the recombinant human-derived TPSB2 protein, expressed by HEK293, with C-6*His labeled tag.

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  • Actividad biológica

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  • Descripciòn

Descripciòn

TPSB2 Protein, Human (HEK293, His) is the recombinant human-derived TPSB2 protein, expressed by HEK293, with C-6*His labeled tag.

Background

TPSB2 is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. It may play a role in innate immunity.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

AAH29356.1 (A19-P275)

Gene ID
Molecular Construction
N-term
TPSB2 (A19-P275)
Accession # AAH29356.1
6*His
C-term
Protein Length

Partial

Synonyms
Tryptase Beta-2; Tryptase-2; Tryptase II; TPSB2; TPS2
AA Sequence

APAPGQALQRVGIVGGQEAPRSKWPWQVSLRVHGPYWMHFCGGSLIHPQWVLTAAHCVGPDVKDLAALRVQLREQHLYYQDQLLPVSRIIVHPQFYTAQIGADIALLELEEPVKVSSHVHTVTLPPASETFPPGMPCWVTGWGDVDNDERLPPPFPLKQVKVPIMENHICDAKYHLGAYTGDDVRIVRDDMLCAGNTRRDSCQGDSGGPLVCKVNGTWLQAGVVSWGEGCAQPNRPGIYTRVTYYLDWIHHYVPKKP

Predicted Molecular Mass
29.6 kDa
Peso molecular

Approximately 30-35 kDa, based on SDS-PAGE under reducing conditions.

Glycosylation
Yes
Pureza
  • ≥ 95%, as determined by reducing SDS-PAGE.
Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Envío

Shipping with dry ice.

Documentación

TPSB2 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Inquiry Information

Nombre del producto:
TPSB2 Protein, Human (HEK293, His)
Cat. No.:
HY-P71125
Cantidad:
MCE Japan Authorized Agent: