TPX2 Protein, Human (sf9, His)
TPX2 Protein, Human (sf9, His) is the recombinant human-derived TPX2, expressed by Sf9 insect cells , with His labeled tag. ,
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
TPX2 Protein, Human (sf9, His) is the recombinant human-derived TPX2, expressed by Sf9 insect cells , with His labeled tag. ,
Background
TPX2 is a spindle assembly factor essential for the normal assembly of mitotic spindles. It is required for proper microtubule assembly during apoptosis and mediates chromatin- and/or kinetochore-dependent microtubule nucleation. TPX2 localizes AURKA to spindle microtubules and activates it by promoting autophosphorylation at Thr-288 while protecting this residue from dephosphorylation. TPX2 is inactivated upon binding to importin-alpha. At the onset of mitosis, GOLGA2 interacts with importin-alpha, releasing TPX2 to activate AURKA kinase and stimulate local microtubule nucleation.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag His
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Accession
Q9ULW0-1 (M1-C747)
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Gene ID22974
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Protein Length
Full Length of Isoform-1
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Synonyms
TPX2; DIL2; Prev. C20orf1; Restricted Expression Proliferation Associated Protein 100; Prev. C20orf2; Restricted Expression Proliferation-Associated Protein 100; HCA519; TPX2, Microtubule-Associated, Homolog (Xenopus Laevis); DIL-2; Preferentially Express
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AA Sequence
MSQVKSSYSYDAPSDFINFSSLDDEGDTQNIDSWFEEKANLENKLLGKNGTGGLFQGKTPLRKANLQQAIVTPLKPVDNTYYKEAEKENLVEQSIPSNACSSLEVEAAISRKTPAQPQRRSLRLSAQKDLEQKEKHHVKMKAKRCATPVIIDEILPSKKMKVSNNKKKPEEEGSAHQDTAEKNASSPEKAKGRHTVPCMPPAKQKFLKSTEEQELEKSMKMQQEVVEMRKKNEEFKKLALAGIGQPVKKSVSQVTKSVDFHFRTDERIKQHPKNQEEYKEVNFTSELRKHPSSPARVTKGCTIVKPFNLSQGKKRTFDETVSTYVPLAQQVEDFHKRTPNRYHLRSKKDDINLLPSKSSVTKICRDPQTPVLQTKHRARAVTCKSTAELEAEELEKLQQYKFKARELDPRILEGGPILPKKPPVKPPTEPIGFDLEIEKRIQERESKKKTEDEHFEFHSRPCPTKILEDVVGVPEKKVLPITVPKSPAFALKNRIRMPTKEDEEEDEPVVIKAQPVPHYGVPFKPQIPEARTVEICPFSFDSRDKERQLQKEKKIKELQKGEVPKFKALPLPHFDTINLPEKKVKNVTQIEPFCLETDRRGALKAQTWKHQLEEELRQQKEAACFKARPNTVISQEPFVPKKEKKSVAEGLSGSLVQEPFQLATEKRAKERQELEKRMAEVEAQKAQQLEEARLQEEEQKKEELARLRRELVHKANPIRKYQGLEIKSSDQPLTVPVSPKFSTRFHC
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Molecular Weight
Approximately 110 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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