TRAIL/TNFSF10 Protein, Human (N-His)

TRAIL/TNFSF10 protein is a cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly TNFRSF11B/OPG. It induces apoptosis, a process that can be mediated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG, which lack the ability to induce apoptosis. TRAIL/TNFSF10 Protein, Human (N-His) is the recombinant human-derived TRAIL/TNFSF10 protein, expressed by E. coli , with N-6*His labeled tag.

TRAIL/TNFSF10 protein, a cytokine, binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly TNFRSF11B/OPG. It induces apoptosis, a process that may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG, which lack the ability to induce apoptosis. Existing as a homotrimer, one TNFSF10 homotrimer interacts with three TNFSF10A monomers, and similarly, one TNFSF10 homotrimer interacts with three TNFSF10B monomers. This intricate interaction underlines the complexity of TRAIL/TNFSF10-mediated apoptotic signaling, showcasing its ability to engage multiple receptors and form distinct molecular configurations for its biological activity.

Measured in a cytotoxicity assay using L929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.The ED₅₀ for this effect is 5.448 ng/mL, corresponding to a specific activity is 1.8355×10⁵ units/mg.

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  Measured in a cytotoxicity assay using L929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.The ED₅₀ for this effect is 5.448 ng/mL, corresponding to a specific activity is 1.8355×10⁵ units/mg.

Human

E. coli

N-6*His

P50591 (V114-G281)

8743  [NCBI]

Partial

VRERGPQRVAAHITGTRGRSNTLSSPNSKNEKALGRKINSWESSRSGHSFLSNLHLRNGELVIHEKGFYYIYSQTYFRFQEEIKENTKNDKQMVQYIYKYTSYPDPILLMKSARNSCWSKDAEYGLYSIYQGGIFELKENDRIFVSVTNEHLIDMDHEASFFGAFLVG

Approximately 22 kDa, based on SDS-PAGE under reducing conditions.

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.