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Transferrin Protein, Pig (HEK293, His)

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Transferrin is an important iron-binding transport protein that binds two Fe(3+) ions, usually with anions such as bicarbonate. Crucially, it transports iron from the site of absorption and heme degradation to the site of storage and utilization. Transferrin Protein, Pig (HEK293, His) is the recombinant pig-derived Transferrin protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Pig
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Transferrin Protein, Pig (HEK293, His)
  • Experimental Validation Results for Transferrin Protein, Pig (HEK293, His)
  • Transferrin Protein, Pig (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Use of MCE Products

Biological Activity

Description

Transferrin is an important iron-binding transport protein that binds two Fe(3+) ions, usually with anions such as bicarbonate. Crucially, it transports iron from the site of absorption and heme degradation to the site of storage and utilization. Transferrin Protein, Pig (HEK293, His) is the recombinant pig-derived Transferrin protein, expressed by HEK293 , with C-His labeled tag.

Background

Transferrins, as iron-binding transport proteins, exhibit the capability to bind two Fe(3+) ions, often in conjunction with the binding of an anion, typically bicarbonate. Functionally, transferrin plays a crucial role in transporting iron from sites of absorption and heme degradation to locations dedicated to storage and utilization within the body. Beyond its primary involvement in iron homeostasis, serum transferrin may play an additional role in stimulating cell proliferation. Structured as a monomer, transferrin's versatility underscores its significance in orchestrating the intricate processes of iron transport and cellular regulation, contributing to essential physiological functions within the organism.

Verified Bioactivity

Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is 22.35 ng/mL, corresponding to a specific activity is 4.47×104 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is 22.35 ng/mL, corresponding to a specific activity is 4.47×104 units/mg.

Technical Parameters

  • Species Pig
  • Source HEK293
  • Tag C-His
  • Accession

    B3CL06 (Q22-T715)

  • Gene ID
    396996  [NCBI]
  • Molecular Construction
    • N-term
    • Transferrin (Q22-T715)
      Accession # B3CL06
    • His
    • C-term
  • Protein Length

    Full Length of Serotransferrin Chain

  • Synonyms

    TF; Epididymis Secretory Sperm Binding Protein Li 71p; Transferrin; CDNA FLJ54029, Highly Similar To Serotransferrin; Serotransferrin; Transferrin, Isoform CRA_c; Beta-1 Metal-Binding Globulin; TF Protein; Siderophilin; HEL-S-71p; PRO1557; TFQTL1; PRO2086

  • AA Sequence

    QKTVRWCTISNQEANKCSSFRENMSKAVKNGPLVSCVKKSSYLDCIKAIRDKEADAVTLDAGLVFEAGLAPYNLKPVVAEFYGQKDNPQTHYYAVAVVKKGSNFQWNQLQGKRSCHTGLGRSAGWIIPMGLLYDQLPEPRKPIEKAVASFFSSSCVPCADPVNFPKLCQQCAGKGAEKCACSNHEPYFGYAGAFNCLKEDAGDVAFVKHSTVLENLPDKADRDQYELLCRDNTRRPVDDYENCYLAQVPSHAVVARSVDGQEDSIWELLNQAQEHFGRDKSPDFQLFSSSHGKDLLFKDSANGFLRIPSKMDSSLYLGYQYVTALRNLREEISPDSSKNECKKVRWCAIGHEETQKCDAWSINSGGKIECVSAENTEDCIAKIVKGEADAMSLDGGYIYIAGKCGLVPVLAENYKTEGENCVNTPEKGYLAVAVVKKSSGPDLNWNNLKGKKSCHTAVDRTAGWNIPMGLLYNKINSCKFDQFFGEGCAPGSQRNSSLCALCIGSERAPGRECLANNHERYYGYTGAFRCLVEKGDVAFVKDQVVQQNTDGKNKDDWAKDLKQMDFELLCQNGAREPVDNAENCHLARAPNHAVVARDDKVTCVAEELLKQQAQFGRHVTDCSSSFCMFKSNTKDLLFRDDTQCLARVGKTTYESYLGADYITAVANLRKCSTSKLLEACTFHSAKNPRVETTT

  • Predicted Molecular Mass

    76.8 kDa

  • Molecular Weight

    Approximately 72 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is 22.35 ng/mL, corresponding to a specific activity is 4.47×104 units/mg.

  • Transferrin Protein, Pig (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Transferrin Protein, Pig (HEK293, His)
  • Experimental Validation Results for Transferrin Protein, Pig (HEK293, His)
  • Transferrin Protein, Pig (HEK293, His) Product Image