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Tryptase beta-2 Protein, Mouse (HEK293, His)

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Based on 1 publication(s) in Google Scholar

Tryptase beta-2, a neutral protease abundant in mast cells, plays a key role in innate immunity.As the primary proteolytic enzyme released during mast cell activation, Tryptase beta-2 contributes significantly to immune functions.Its involvement underscores its importance in mediating cellular responses linked to innate immunity, highlighting its role in the intricate defense mechanisms of the immune system.Tryptase beta-2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Tryptase beta-2 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for Tryptase beta-2 Protein, Mouse (HEK293, His)
  • Tryptase beta-2 Protein, Mouse (HEK293, His) Product Image
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Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

Tryptase beta-2, a neutral protease abundant in mast cells, plays a key role in innate immunity.As the primary proteolytic enzyme released during mast cell activation, Tryptase beta-2 contributes significantly to immune functions.Its involvement underscores its importance in mediating cellular responses linked to innate immunity, highlighting its role in the intricate defense mechanisms of the immune system.Tryptase beta-2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Tryptase beta-2 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

Tryptase beta-2, a neutral protease primarily found in mast cells, assumes a significant role in innate immunity. It acts as the major proteolytic enzyme released during the activation-degranulation response of mast cells, contributing to their immune functions. The involvement of Tryptase beta-2 underscores its importance in mediating cellular responses associated with innate immunity, emphasizing its role in the complex and dynamic defense mechanisms of the immune system.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-6*His
  • Accession

    P21845 (A22-S276)

  • Gene ID
    17229  [NCBI]
  • Molecular Construction
    • N-term
    • TPSB2 (A22-S276)
      Accession # P21845
    • 6*His
    • C-term
  • Protein Length

    Full Length of Mature Protein (with Propeptide)

  • Synonyms

    TPSB2; Tryptase Beta III; Tryptase Beta 2; Tryptase Beta II; Testicular Tissue Protein Li 163; TPSB2 Protein; Tryptase Beta-2; Tryptase II; TPS2; Tryptase-2; Tryptase Beta 2 (Gene/Pseudogene); TryptaseB; Mast Cell Tryptase Beta III; TryptaseC; Mast Cell T

  • AA Sequence

    APRPANQRVGIVGGHEASESKWPWQVSLRFKLNYWIHFCGGSLIHPQWVLTAAHCVGPHIKSPQLFRVQLREQYLYYGDQLLSLNRIVVHPHYYTAEGGADVALLELEVPVNVSTHIHPISLPPASETFPPGTSCWVTGWGDIDNDEPLPPPYPLKQVKVPIVENSLCDRKYHTGLYTGDDFPIVHDGMLCAGNTRRDSCQGDSGGPLVCKVKGTWLQAGVVSWGEGCAQPNKPGIYTRVTYYLDWIHRYVPEHS

  • Predicted Molecular Mass

    29.3 kDa

  • Molecular Weight

    Approximately 32-38 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM PB, 50% glycerin, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • Tryptase beta-2 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Tryptase beta-2 Protein, Mouse (HEK293, His)
  • Tryptase beta-2 Protein, Mouse (HEK293, His) Product Image