TSG-6 Protein, Human (His)

The TSG-6 protein is a major extracellular matrix regulator that catalyzes the transfer of heavy chains to hyaluronic acid to form hyaluronic acid-HC oligomers that are critical for tissue remodeling and oocyte fertilization. It assembles hyaluronic acid in the pericellular matrix, which acts as a platform for receptor aggregation. TSG-6 Protein, Human (His) is the recombinant human-derived TSG-6 protein, expressed by E. coli , with N-6*His labeled tag.

The TSG-6 protein is a major regulator of extracellular matrix organization during tissue remodeling. It catalyzes the transfer of a heavy chain from the inter-alpha-inhibitor complex to hyaluronan, facilitating the formation of hyaluronan-HC oligomers and a hyaluronan-rich matrix surrounding the cumulus cell-oocyte complex, which is essential for oocyte fertilization. Additionally, TSG-6 assembles hyaluronan in pericellular matrices that act as platforms for receptor clustering and signaling. It also plays a role in immune response modulation, by altering hyaluronan binding to CD44 on vascular endothelium and facilitating leukocyte rolling. Furthermore, TSG-6 modulates the interaction of chemokines with extracellular matrix components and proteoglycans on endothelial cell surfaces, thereby preventing chemokine gradient formation. It also limits excessive neutrophil recruitment at inflammatory sites by antagonizing the association of CXCL8 with glycosaminoglycans on vascular endothelium. TSG-6 has a role in osteogenesis and bone remodeling as well, inhibiting the differentiation of mesenchymal stem cells to osteoblasts and protecting against bone erosion during inflammation by inhibiting osteoclast activation.

Human

E. coli

N-6*His

P98066 (W18-L277)

7130

Full Length of Mature Protein

WGFKDGIFHNSIWLERAAGVYHREARSGKYKLTYAEAKAVCEFEGGHLATYKQLEAARKIGFHVCAAGWMAKGRVGYPIVKPGPNCGFGKTGIIDYGIRLNRSERWDAYCYNPHAKECGGVFTDPKQIFKSPGFPNEYEDNQICYWHIRLKYGQRIHLSFLDFDLEDDPGCLADYVEIYDSYDDVHGFVGRYCGDELPDDIISTGNVMTLKFLSDASVTAGGFQIKYVAMDPVSKSSQGKNTSTTSTGNKNFLAGRFSHL

Approximately 35 kDa, based on SDS-PAGE under reducing conditions.

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
2.Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.