UAP1 Protein, Human (P.pastoris, His)
UAP1 protein functions as an enzyme that catalyzes the conversion of UTP and GlcNAc-1-P into UDP-GlcNAc, as well as UTP and GalNAc-1-P into UDP-GalNAc. Notably, isoform AGX1 exhibits 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 displays 8 times more activity towards GlcNAc-1-P, highlighting distinct substrate preferences between the isoforms. UAP1 Protein, Human (P.pastoris, His) is the recombinant human-derived UAP1 protein, expressed by P. pastoris , with N-His labeled tag.
- Species: Human
- Source: P. pastoris
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
UAP1 protein functions as an enzyme that catalyzes the conversion of UTP and GlcNAc-1-P into UDP-GlcNAc, as well as UTP and GalNAc-1-P into UDP-GalNAc. Notably, isoform AGX1 exhibits 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 displays 8 times more activity towards GlcNAc-1-P, highlighting distinct substrate preferences between the isoforms. UAP1 Protein, Human (P.pastoris, His) is the recombinant human-derived UAP1 protein, expressed by P. pastoris , with N-His labeled tag.
Background
Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) and uridine diphosphate-N-acetylgalactosamine (UDP-GalNAc) are crucial nucleotide sugars involved in glycosylation processes, and the UAP1 protein plays a central role in their synthesis. UAP1 converts uridine triphosphate (UTP) in conjunction with either GlcNAc-1-P or GalNAc-1-P to generate UDP-GlcNAc or UDP-GalNAc, respectively. Interestingly, isoform AGX1 of UAP1 exhibits 2 to 3 times higher activity towards GalNAc-1-P, emphasizing its preference for galactosamine, while isoform AGX2 displays 8 times greater activity towards GlcNAc-1-P, highlighting its specificity for N-acetylglucosamine. The differential activities of these isoforms underscore the versatility of UAP1 in contributing to the generation of distinct nucleotide sugars, crucial for various glycosylation events in cellular processes.
Technical Parameters
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Species Human
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Source P. pastoris
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Tag N-His
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Accession
Q16222 (M1-I522)
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Molecular Construction
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N-term
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His
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UAP1 (M1-I522)
Accession # Q16222 -
C-term
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Protein Length
Full Length
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Synonyms
UAP1; SPAG2; UDP-N-acetylhexosamine pyrophosphorylase; Antigen X; AGX; Protein-pyrophosphorylation enzyme; EC 2.7.4.-; Sperm-associated antigen 2; UDP-N-acetylgalactosamine pyrophosphorylase; EC 2.7.7.83; UDP-N-acetylglucosamine pyrophosphorylase; EC 2.7.
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AA Sequence
MNINDLKLTLSKAGQEHLLRFWNELEEAQQVELYAELQAMNFEELNFFFQKAIEGFNQSSHQKNVDARMEPVPREVLGSATRDQDQLQAWESEGLFQISQNKVAVLLLAGGQGTRLGVAYPKGMYDVGLPSRKTLFQIQAERILKLQQVAEKYYGNKCIIPWYIMTSGRTMESTKEFFTKHKYFGLKKENVIFFQQGMLPAMSFDGKIILEEKNKVSMAPDGNGGLYRALAAQNIVEDMEQRGIWSIHVYCVDNILVKVADPRFIGFCIQKGADCGAKVVEKTNPTEPVGVVCRVDGVYQVVEYSEISLATAQKRSSDGRLLFNAGNIANHFFTVPFLRDVVNVYEPQLQHHVAQKKIPYVDTQGQLIKPDKPNGIKMEKFVFDIFQFAKKFVVYEVLREDEFSPLKNADSQNGKDNPTTARHALMSLHHCWVLNAGGHFIDENGSRLPAIPRSATNGKSETITADVNHNLKDANDVPIQCEISPLISYAGEGLESYVADKEFHAPLIIDENGVHELVKNGI
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Predicted Molecular Mass
60.8 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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