UBE2A Protein, Human (GST-His)

UBE2A cooperates with the E3 enzyme BRE1 to promote the attachment of ubiquitin to proteins, specifically histone H2B at "Lys-120", forming H2BK120ub1. This modification serves as a tag for transcriptional activation, RNA polymerase II elongation, telomeric silencing, and is critical for the formation of H3K4me and H3K79me. UBE2A Protein, Human (GST-His) is the recombinant human-derived UBE2A protein, expressed by E. coli , with N-6*His, N-GST labeled tag. The total length of UBE2A Protein, Human (GST-His) is 152 a.a., with molecular weight of ~52.0 kDa.

UBE2A, as an integral component of the ubiquitin-proteasome system, serves as a conduit for ubiquitin transfer from the E1 complex to target proteins, catalyzing their covalent attachment. Collaborating with the E3 enzyme BRE1 (RNF20 and/or RNF40), UBE2A plays a pivotal role in transcriptional regulation by orchestrating the monoubiquitination of histone H2B at 'Lys-120,' resulting in the formation of H2BK120ub1. This modification serves as a distinctive mark for epigenetic transcriptional activation, RNA polymerase II elongation, telomeric silencing, and is essential for the subsequent formation of H3K4me and H3K79me. In addition to its role in histone ubiquitination, UBE2A exhibits catalytic activity in vitro, mediating both 'Lys-11' and 'Lys-48'-linked polyubiquitination. Moreover, UBE2A is indispensable for the postreplication repair of UV-damaged DNA, highlighting its multifaceted involvement in fundamental cellular processes.

E2-ubiquitin conjugation was detected by whether the DTT-sensitive thioester linkage can form or not.

Human

E. coli

N-6*His;N-GST

P49459-1 (M1-C152)

7319  [NCBI]

MSTPARRRLMRDFKRLQEDPPAGVSGAPSENNIMVWNAVIFGPEGTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWRDC

Approximately 52.0 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT, 10% Glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.