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UBE2A Protein, Human (GST-His)

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Based on 1 Customer Validation

UBE2A cooperates with the E3 enzyme BRE1 to promote the attachment of ubiquitin to proteins, specifically histone H2B at "Lys-120", forming H2BK120ub1. This modification serves as a tag for transcriptional activation, RNA polymerase II elongation, telomeric silencing, and is critical for the formation of H3K4me and H3K79me. UBE2A Protein, Human (GST-His) is the recombinant human-derived UBE2A protein, expressed by E. coli , with N-6*His, N-GST labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for UBE2A Protein, Human (GST-His)
  • UBE2A Protein, Human (GST-His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

UBE2A cooperates with the E3 enzyme BRE1 to promote the attachment of ubiquitin to proteins, specifically histone H2B at "Lys-120", forming H2BK120ub1. This modification serves as a tag for transcriptional activation, RNA polymerase II elongation, telomeric silencing, and is critical for the formation of H3K4me and H3K79me. UBE2A Protein, Human (GST-His) is the recombinant human-derived UBE2A protein, expressed by E. coli , with N-6*His, N-GST labeled tag.

Background

UBE2A, as an integral component of the ubiquitin-proteasome system, serves as a conduit for ubiquitin transfer from the E1 complex to target proteins, catalyzing their covalent attachment. Collaborating with the E3 enzyme BRE1 (RNF20 and/or RNF40), UBE2A plays a pivotal role in transcriptional regulation by orchestrating the monoubiquitination of histone H2B at 'Lys-120,' resulting in the formation of H2BK120ub1. This modification serves as a distinctive mark for epigenetic transcriptional activation, RNA polymerase II elongation, telomeric silencing, and is essential for the subsequent formation of H3K4me and H3K79me. In addition to its role in histone ubiquitination, UBE2A exhibits catalytic activity in vitro, mediating both 'Lys-11' and 'Lys-48'-linked polyubiquitination. Moreover, UBE2A is indispensable for the postreplication repair of UV-damaged DNA, highlighting its multifaceted involvement in fundamental cellular processes.

Verified Bioactivity

E2-ubiquitin conjugation was detected by whether the DTT-sensitive thioester linkage can form or not.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-6*His;N-GST
  • Accession

    P49459-1 (M1-C152)

  • Gene ID
    7319  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His-GST
    • UBE2A (M1-C152)
      Accession # P49459-1
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    UBE2A; Ubiquitin Conjugating Enzyme E2 A; HHR6A; RAD6A; UBC2; HR6A; Ubiquitin-Conjugating Enzyme E2 A; E2 Ubiquitin-Conjugating Enzyme A; Ubiquitin-Conjugating Enzyme E2A; Ubiquitin Carrier Protein A; Ubiquitin-Protein Ligase A; RAD6 Homolog A; Ubiquitin-

  • AA Sequence

    MSTPARRRLMRDFKRLQEDPPAGVSGAPSENNIMVWNAVIFGPEGTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWRDC

  • Predicted Molecular Mass

    49 kDa

  • Molecular Weight

    Approximately 45 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT, 10% glycerol, pH 7.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
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MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • UBE2A Protein, Human (GST-His) Product Image
    1/16
  • Experimental Validation Results for UBE2A Protein, Human (GST-His)
  • UBE2A Protein, Human (GST-His) Product Image