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UBE2K Protein, Human (GST)

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Based on 1 Customer Validation

UBE2K protein is an important component of the ubiquitin-proteasome system and functions as an E2 ubiquitin-conjugating enzyme, which is critical for attaching ubiquitin to different substrates. In vitro, UBE2K catalyzes "Lys-48" linked polyubiquitin chains, specifically in the presence or absence of the BRCA1-BARD1 E3 ligase complex. UBE2K Protein, Human (GST) is the recombinant human-derived UBE2K protein, expressed by E. coli , with N-GST labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for UBE2K Protein, Human (GST)
  • UBE2K Protein, Human (GST) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

UBE2K protein is an important component of the ubiquitin-proteasome system and functions as an E2 ubiquitin-conjugating enzyme, which is critical for attaching ubiquitin to different substrates. In vitro, UBE2K catalyzes "Lys-48" linked polyubiquitin chains, specifically in the presence or absence of the BRCA1-BARD1 E3 ligase complex. UBE2K Protein, Human (GST) is the recombinant human-derived UBE2K protein, expressed by E. coli , with N-GST labeled tag.

Background

UBE2K, a pivotal component of the ubiquitin-proteasome system, serves as an E2 ubiquitin-conjugating enzyme, playing a crucial role in the covalent attachment of ubiquitin to diverse substrate proteins. In vitro studies reveal its capability to catalyze the synthesis of 'Lys-48'-linked polyubiquitin chains, particularly in the presence or absence of the BRCA1-BARD1 E3 ubiquitin-protein ligase complex. Notably, UBE2K is implicated in mediating the selective degradation of short-lived and aberrant proteins, participating in processes such as endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Furthermore, UBE2K demonstrates ubiquitination activity towards various targets, including huntingtin, p53/TP53, and potentially NF-kappa-B. Additionally, its involvement in viral infections is suggested, where UBE2K may contribute to the degradation of MHC class I heavy chains and participate in the HPV E7 protein-dependent degradation of RB1.

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-GST
  • Accession

    P61086-1 (M1-N200)

  • Gene ID
    3093  [NCBI]
  • Molecular Construction
    • N-term
    • GST
    • UBE2K (M1-N200)
      Accession # P61086-1
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    UBE2K; Ubiquitin Conjugating Enzyme E2 K; HIP2; HYP G; UBC1; Ubiquitin‑Conjugating Enzyme E2K (UBC1 Homolog, Yeast); Ubiquitin‑Conjugating Enzyme E2(25K); Ubiquitin‑Conjugating Enzyme E2 K; E2 Ubiquitin‑Conjugating Enzyme K; Huntingtin‑Interacting Protein

  • AA Sequence

    MANIAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIAGPPDTPYEGGRYQLEIKIPETYPFNPPKVRFITKIWHPNISSVTGAICLDILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYAGAPVSSPEYTKKIENLCAMGFDRNAVIVALSSKSWDVETATELLLSN

  • Predicted Molecular Mass

    48.7 kDa

  • Molecular Weight

    Approximately 45 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

1.Supplied as a 0.22 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT, 10% glycerol, pH 7.5.
2.Supplied as a 0.22 μm filtered solution of 20mM PBS, 6% Trehalose, 0.02% Tween 20, pH 7.2.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • UBE2K Protein, Human (GST) Product Image
    1/16
  • Experimental Validation Results for UBE2K Protein, Human (GST)
  • UBE2K Protein, Human (GST) Product Image