1. Recombinant Proteins
  2. Ubiquitin Related Proteins
  3. Ubiquitin Enzymes
  4. E2 Enzymes
  5. Ubiquitin-Conjugating Enzyme E2 K
  6. UBE2K Protein, Human (GST)

UBE2K Protein, Human (GST)

Cat. No.: HY-P71405
COA Handling Instructions

UBE2K protein is an important component of the ubiquitin-proteasome system and functions as an E2 ubiquitin-conjugating enzyme, which is critical for attaching ubiquitin to different substrates. In vitro, UBE2K catalyzes "Lys-48" linked polyubiquitin chains, specifically in the presence or absence of the BRCA1-BARD1 E3 ligase complex. UBE2K Protein, Human (GST) is the recombinant human-derived UBE2K protein, expressed by E. coli , with N-GST labeled tag.

For research use only. We do not sell to patients.

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50 μg $90 In-stock
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Description

UBE2K protein is an important component of the ubiquitin-proteasome system and functions as an E2 ubiquitin-conjugating enzyme, which is critical for attaching ubiquitin to different substrates. In vitro, UBE2K catalyzes "Lys-48" linked polyubiquitin chains, specifically in the presence or absence of the BRCA1-BARD1 E3 ligase complex. UBE2K Protein, Human (GST) is the recombinant human-derived UBE2K protein, expressed by E. coli , with N-GST labeled tag.

Background

UBE2K, a pivotal component of the ubiquitin-proteasome system, serves as an E2 ubiquitin-conjugating enzyme, playing a crucial role in the covalent attachment of ubiquitin to diverse substrate proteins. In vitro studies reveal its capability to catalyze the synthesis of 'Lys-48'-linked polyubiquitin chains, particularly in the presence or absence of the BRCA1-BARD1 E3 ubiquitin-protein ligase complex. Notably, UBE2K is implicated in mediating the selective degradation of short-lived and aberrant proteins, participating in processes such as endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Furthermore, UBE2K demonstrates ubiquitination activity towards various targets, including huntingtin, p53/TP53, and potentially NF-kappa-B. Additionally, its involvement in viral infections is suggested, where UBE2K may contribute to the degradation of MHC class I heavy chains and participate in the HPV E7 protein-dependent degradation of RB1.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

E. coli

Tag

N-GST

Accession

P61086-1 (M1-N200)

Gene ID
Molecular Construction
N-term
GST
UBE2K (M1-N200)
Accession # P61086-1
C-term
Synonyms
Ubiquitin-Conjugating Enzyme E2 K; Huntingtin-Interacting Protein 2; HIP-2; Ubiquitin Carrier Protein; Ubiquitin-Conjugating Enzyme E2-25 kDa; Ubiquitin-Conjugating Enzyme E2(25K); Ubiquitin-Conjugating Enzyme E2-25K; Ubiquitin-Protein Ligase; UBE2K; HIP2; LIG
AA Sequence

MANIAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIAGPPDTPYEGGRYQLEIKIPETYPFNPPKVRFITKIWHPNISSVTGAICLDILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYAGAPVSSPEYTKKIENLCAMGFDRNAVIVALSSKSWDVETATELLLSN

Molecular Weight

Approximately 45.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT, 10% Glycerol, pH 7.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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UBE2K Protein, Human (GST)
Cat. No.:
HY-P71405
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