UROIIIS Protein, Human (His)
The UROIIIS protein is a key catalyst in biochemical processes, specifically catalyzing the cyclization of hydroxymethyl bixane, a linear tetrapyrrole, to form macrocyclic uroporphyrinogen III. This enzymatic step serves as a critical branching point leading to various subpathways responsible for the synthesis of different porphyrins. UROIIIS Protein, Human (His) is the recombinant human-derived UROIIIS protein, expressed by E. coli , with C-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The UROIIIS protein is a key catalyst in biochemical processes, specifically catalyzing the cyclization of hydroxymethyl bixane, a linear tetrapyrrole, to form macrocyclic uroporphyrinogen III. This enzymatic step serves as a critical branching point leading to various subpathways responsible for the synthesis of different porphyrins. UROIIIS Protein, Human (His) is the recombinant human-derived UROIIIS protein, expressed by E. coli , with C-6*His labeled tag.
Background
UROIIIS, or uroporphyrinogen-III synthase, plays a pivotal role in tetrapyrrole biosynthesis by catalyzing the cyclization of hydroxymethylbilane to form the macrocyclic uroporphyrinogen III. This enzymatic step represents a critical branch point, giving rise to various sub-pathways leading to the diverse array of porphyrins. Porphyrins, synthesized through this process, serve as essential cofactors for numerous enzymes involved in diverse cellular processes. These processes include methionine synthesis, where porphyrins act as cofactors for enzymes related to vitamin B12 metabolism, as well as oxygen transport, where porphyrins, particularly heme, play a crucial role in binding and transporting oxygen in hemoglobin. It has to highlight UROIIIS's central role in porphyrin biosynthesis and its importance in supporting various biological processes through the generation of essential cofactors.
Technical Parameters
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Species Human
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Source E. coli
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Tag C-6*His
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Accession
P10746 (M1-C265)
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Molecular Construction
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N-term
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UROIIIS (M1-C265)
Accession # P10746 -
6*His
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C-term
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Protein Length
Full Length
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Synonyms
UROS; Uroporphyrinogen III Synthase; Uroporphyrinogen-III Cosynthase; Uroporphyrinogen-III Synthase; Hydroxymethylbilane Hydrolyase [Cyclizing]; UROIII; Congenital Erythropoietic Porphyria; Hydroxymethylbilane Hydrolyase; UROS Protein; Mgu
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AA Sequence
MKVLLLKDAKEDDCGQDPYIRELGLYGLEATLIPVLSFEFLSLPSFSEKLSHPEDYGGLIFTSPRAVEAAELCLEQNNKTEVWERSLKEKWNAKSVYVVGNATASLVSKIGLDTEGETCGNAEKLAEYICSRESSALPLLFPCGNLKREILPKALKDKGIAMESITVYQTVAHPGIQGNLNSYYSQQGVPASITFFSPSGLTYSLKHIQELSGDNIDQIKFAAIGPTTARALAAQGLPVSCTAESPTPQALATGIRKALQPHGCC
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Predicted Molecular Mass
29.7 kDa
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Molecular Weight
Approximately 29 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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