UROIIIS Protein, Human (His)

The UROIIIS protein is a key catalyst in biochemical processes, specifically catalyzing the cyclization of hydroxymethyl bixane, a linear tetrapyrrole, to form macrocyclic uroporphyrinogen III. This enzymatic step serves as a critical branching point leading to various subpathways responsible for the synthesis of different porphyrins. UROIIIS Protein, Human (His) is the recombinant human-derived UROIIIS protein, expressed by E. coli , with C-6*His labeled tag. The total length of UROIIIS Protein, Human (His) is 265 a.a., with molecular weight of ~29.0 kDa.

UROIIIS, or uroporphyrinogen-III synthase, plays a pivotal role in tetrapyrrole biosynthesis by catalyzing the cyclization of hydroxymethylbilane to form the macrocyclic uroporphyrinogen III. This enzymatic step represents a critical branch point, giving rise to various sub-pathways leading to the diverse array of porphyrins. Porphyrins, synthesized through this process, serve as essential cofactors for numerous enzymes involved in diverse cellular processes. These processes include methionine synthesis, where porphyrins act as cofactors for enzymes related to vitamin B12 metabolism, as well as oxygen transport, where porphyrins, particularly heme, play a crucial role in binding and transporting oxygen in hemoglobin. It has to highlight UROIIIS's central role in porphyrin biosynthesis and its importance in supporting various biological processes through the generation of essential cofactors.

Human

E. coli

C-6*His

P10746 (M1-C265)

7390  [NCBI]

MKVLLLKDAKEDDCGQDPYIRELGLYGLEATLIPVLSFEFLSLPSFSEKLSHPEDYGGLIFTSPRAVEAAELCLEQNNKTEVWERSLKEKWNAKSVYVVGNATASLVSKIGLDTEGETCGNAEKLAEYICSRESSALPLLFPCGNLKREILPKALKDKGIAMESITVYQTVAHPGIQGNLNSYYSQQGVPASITFFSPSGLTYSLKHIQELSGDNIDQIKFAAIGPTTARALAAQGLPVSCTAESPTPQALATGIRKALQPHGCC

Approximately 29.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.