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USP11 Protein, Human (sf9, GST)

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The USP11 protein is a multifunctional protease that selectively removes ubiquitin from target proteins and polyubiquitin chains, thereby hindering their proteasomal degradation. It shows a preference for “Lys-6” and “Lys-63” linked ubiquitin chains and less activity towards other linkages. USP11 Protein, Human (sf9, GST) is the recombinant human-derived USP11 protein, expressed by sf9 insect cells , with N-GST labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • USP11 Protein, Human (sf9, GST) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The USP11 protein is a multifunctional protease that selectively removes ubiquitin from target proteins and polyubiquitin chains, thereby hindering their proteasomal degradation. It shows a preference for “Lys-6” and “Lys-63” linked ubiquitin chains and less activity towards other linkages. USP11 Protein, Human (sf9, GST) is the recombinant human-derived USP11 protein, expressed by sf9 insect cells , with N-GST labeled tag.

Background

USP11 protein functions as a protease with the capability to remove conjugated ubiquitin from target proteins and polyubiquitin chains, inhibiting their degradation by the proteasome. Exhibiting a preference for 'Lys-6' and 'Lys-63'-linked ubiquitin chains, USP11 shows lower activity towards 'Lys-11' and 'Lys-33'-linked chains, and minimal activity with 'Lys-27', 'Lys-29', and 'Lys-48'-linked chains in vitro. Beyond its proteolytic role, USP11 is integral to the regulation of pathways leading to NF-kappa-B activation and plays a crucial role in DNA repair following double-stranded DNA breaks. Additionally, it acts as a chromatin regulator through association with the Polycomb group (PcG) multiprotein PRC1-like complex, potentially by deubiquitinating components of this complex. USP11's involvement in cell proliferation is highlighted by its ability to deubiquitinate phosphorylated E2F1, further emphasizing its multifaceted role in cellular processes.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag N-GST
  • Accession

    P51784 (A2-N963)

  • Gene ID
    /
  • Molecular Construction
    • N-term
    • GST
    • USP11 (A2-N963)
      Accession # P51784
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    USP11; Ubiquitin Specific Peptidase 11; UHX1; Ubiquitin Carboxyl-Terminal Hydrolase 11; Deubiquitinating Enzyme 11; Ubiquitin Thioesterase 11; Ubiquitin Carboxyl-Terminal Hydrolase, X-Linked; Ubiquitin-Specific Processing Protease 11; Ubiquitin-Specific-P

  • AA Sequence

    AVAPRLFGGLCFRFRDQNPEVAVEGRLPISHSCVGCRRERTAMATVAANPAAAAAAVAAAAAVTEDREPQHEELPGLDSQWRQIENGESGRERPLRAGESWFLVEKHWYKQWEAYVQGGDQDSSTFPGCINNATLFQDEINWRLKEGLVEGEDYVLLPAAAWHYLVSWYGLEHGQPPIERKVIELPNIQKVEVYPVELLLVRHNDLGKSHTVQFSHTDSIGLVLRTARERFLVEPQEDTRLWAKNSEGSLDRLYDTHITVLDAALETGQLIIMETRKKDGTWPSAQLHVMNNNMSEEDEDFKGQPGICGLTNLGNTCFMNSALQCLSNVPQLTEYFLNNCYLEELNFRNPLGMKGEIAEAYADLVKQAWSGHHRSIVPHVFKNKVGHFASQFLGYQQHDSQELLSFLLDGLHEDLNRVKKKEYVELCDAAGRPDQEVAQEAWQNHKRRNDSVIVDTFHGLFKSTLVCPDCGNVSVTFDPFCYLSVPLPISHKRVLEVFFIPMDPRRKPEQHRLVVPKKGKISDLCVALSKHTGISPERMMVADVFSHRFYKLYQLEEPLSSILDRDDIFVYEVSGRIEAIEGSREDIVVPVYLRERTPARDYNNSYYGLMLFGHPLLVSVPRDRFTWEGLYNVLMYRLSRYVTKPNSDDEDDGDEKEDDEEDKDDVPGPSTGGSLRDPEPEQAGPSSGVTNRCPFLLDNCLGTSQWPPRRRRKQLFTLQTVNSNGTSDRTTSPEEVHAQPYIAIDWEPEMKKRYYDEVEAEGYVKHDCVGYVMKKAPVRLQECIELFTTVETLEKENPWYCPSCKQHQLATKKLDLWMLPEILIIHLKRFSYTKFSREKLDTLVEFPIRDLDFSEFVIQPQNESNPELYKYDLIAVSNHYGGMRDGHYTTFACNKDSGQWHYFDDNSVSPVNENQIESKAAYVLFYQRQDVARRLLSPAGSSGAPASPACSSPPSSEFMDVN

  • Predicted Molecular Mass

    136.3 kDa

  • Molecular Weight

    Approximately 136 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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