USP15 Protein, Human (sf9, GST)
Based on 1 Customer Validation
USP15 is a hydrolase that complexly regulates cellular processes by deubiquitinating various target proteins in pathways such as TGF-β receptor, NF-κ-B, and RNF41/NRDP1-PRKN. In TGF-β signaling, it promotes R-SMAD deubiquitination or stabilizes TGFBR1, thereby enhancing pathway activity. USP15 Protein, Human (sf9, GST) is the recombinant human-derived USP15 protein, expressed by Sf9 insect cells , with N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
USP15 is a hydrolase that complexly regulates cellular processes by deubiquitinating various target proteins in pathways such as TGF-β receptor, NF-κ-B, and RNF41/NRDP1-PRKN. In TGF-β signaling, it promotes R-SMAD deubiquitination or stabilizes TGFBR1, thereby enhancing pathway activity. USP15 Protein, Human (sf9, GST) is the recombinant human-derived USP15 protein, expressed by Sf9 insect cells , with N-GST labeled tag.
Background
USP15, a hydrolase, plays a crucial role in cellular regulation by removing conjugated ubiquitin from various target proteins, influencing pathways such as TGF-beta receptor signaling, NF-kappa-B, and RNF41/NRDP1-PRKN pathways. Its involvement in the TGF-beta receptor signaling pathway is multifaceted; it may promote deubiquitination of monoubiquitinated R-SMADs, alleviating their inhibition and activating TGF-beta target genes, or it may mediate deubiquitination and stabilization of TGFBR1, enhancing TGF-beta signaling. USP15 exhibits versatile deubiquitination capabilities, acting on monoubiquitinated substrates and different types of polyubiquitin chains. Additionally, it acts as an inhibitor of mitophagy by counteracting parkin's action, hydrolyzing polyubiquitin chains on target proteins such as MFN2. USP15 is also involved in endosome organization, NF-kappa-B regulation, and negative modulation of antifungal immunity through deubiquitination of various substrates. Furthermore, in microbial infection scenarios, USP15 protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin-proteasome pathway.
Verified Bioactivity
The fundamental role of USP15 is specific removal of ubiquitin from substrates. USP15 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535nM emission light under the excitation condition of 485nM. The signal of which can be quickly and reliably captured using a microplate reader. The concentration of USP15 used is 0.2 nM, and the reaction lasts for 30 minutes. Under these conditions, the signal-to-noise ratio (S/N) of the signal box is greater than or equal to 10.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-GST
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Accession
Q9Y4E8 (M1-N981)
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Gene ID9958
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Molecular Construction
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N-term
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6*His-SUMO
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USP15 (A2-N981)
Accession # Q9Y4E8 -
C-term
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Protein Length
Full Length
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Synonyms
USP15; Ubiquitin Specific Peptidase 15; KIAA0529; UNPH4; Ubiquitin-Specific-Processing Protease 15; Ubiquitin Carboxyl-Terminal Hydrolase 15; Deubiquitinating Enzyme 15; Ubiquitin Thioesterase 15; Ubiquitin Specific Protease 15; Ubiquitin Thiolesterase 15
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AA Sequence
MAEGGAADLDTQRSDIATLLKTSLRKGDTWYLVDSRWFKQWKKYVGFDSWDKYQMGDQNVYPGPIDNSGLLKDGDAQSLKEHLIDELDYILLPTEGWNKLVSWYTLMEGQEPIARKVVEQGMFVKHCKVEVYLTELKLCENGNMNNVVTRRFSKADTIDTIEKEIRKIFSIPDEKETRLWNKYMSNTFEPLNKPDSTIQDAGLYQGQVLVIEQKNEDGTWPRGPSTPKSPGASNFSTLPKISPSSLSNNYNNMNNRNVKNSNYCLPSYTAYKNYDYSEPGRNNEQPGLCGLSNLGNTCFMNSAIQCLSNTPPLTEYFLNDKYQEELNFDNPLGMRGEIAKSYAELIKQMWSGKFSYVTPRAFKTQVGRFAPQFSGYQQQDCQELLAFLLDGLHEDLNRIRKKPYIQLKDADGRPDKVVAEEAWENHLKRNDSIIVDIFHGLFKSTLVCPECAKISVTFDPFCYLTLPLPMKKERTLEVYLVRMDPLTKPMQYKVVVPKIGNILDLCTALSALSGIPADKMIVTDIYNHRFHRIFAMDENLSSIMERDDIYVFEININRTEDTEHVIIPVCLREKFRHSSYTHHTGSSLFGQPFLMAVPRNNTEDKLYNLLLLRMCRYVKISTETEETEGSLHCCKDQNINGNGPNGIHEEGSPSEMETDEPDDESSQDQELPSENENSQSEDSVGGDNDSENGLCTEDTCKGQLTGHKKRLFTFQFNNLGNTDINYIKDDTRHIRFDDRQLRLDERSFLALDWDPDLKKRYFDENAAEDFEKHESVEYKPPKKPFVKLKDCIELFTTKEKLGAEDPWYCPNCKEHQQATKKLDLWSLPPVLVVHLKRFSYSRYMRDKLDTLVDFPINDLDMSEFLINPNAGPCRYNLIAVSNHYGGMGGGHYTAFAKNKDDGKWYYFDDSSVSTASEDQIVSKAAYVLFYQRQDTFSGTGFFPLDRETKGASAATGIPLESDEDSNDNDNDIENENCMHTN
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Predicted Molecular Mass
138.9 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (242 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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