USP15 Protein, Human (sf9)

USP15 is a hydrolase that complexly regulates cellular processes by deubiquitinating various target proteins in pathways such as TGF-β receptor, NF-κ-B, and RNF41/NRDP1-PRKN. In TGF-β signaling, it promotes R-SMAD deubiquitination or stabilizes TGFBR1, thereby enhancing pathway activity. USP15 Protein, Human (sf9) is the recombinant human-derived USP15 protein, expressed by Sf9 insect cells , with tag free. The total length of USP15 Protein, Human (sf9) is 980 a.a. .

USP15, a hydrolase, plays a crucial role in cellular regulation by removing conjugated ubiquitin from various target proteins, influencing pathways such as TGF-beta receptor signaling, NF-kappa-B, and RNF41/NRDP1-PRKN pathways. Its involvement in the TGF-beta receptor signaling pathway is multifaceted; it may promote deubiquitination of monoubiquitinated R-SMADs, alleviating their inhibition and activating TGF-beta target genes, or it may mediate deubiquitination and stabilization of TGFBR1, enhancing TGF-beta signaling. USP15 exhibits versatile deubiquitination capabilities, acting on monoubiquitinated substrates and different types of polyubiquitin chains. Additionally, it acts as an inhibitor of mitophagy by counteracting parkin's action, hydrolyzing polyubiquitin chains on target proteins such as MFN2. USP15 is also involved in endosome organization, NF-kappa-B regulation, and negative modulation of antifungal immunity through deubiquitination of various substrates. Furthermore, in microbial infection scenarios, USP15 protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin-proteasome pathway.

The fundamental role of USP15 is specific removal of ubiquitin from substrates. USP15 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535 nM emission light under the excitation condition of 485 nM. The signal of which can be quickly and reliably captured using a microplate reader.

Human

Sf9 insect cells

Tag Free

Q9Y4E8 (A2-N981)

9958

Approximately 112.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, 200 mM NaCl, 20% glycerol, 1 mM DTT, pH 7.5.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.