USP21 Protein, Human (356a.a)

For research use only. We do not sell to patients.

Size	Price	Stock	Quantity
10 μg	$100	In-stock	Estimated Time of Arrival: December 31
50 μg		Get quote
100 μg		Get quote

* Please select Quantity before adding items.

Biological Activity	The fundamental role of USP21 is specific removal of ubiquitin from substrates. USP21 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535 nM emission light under the excitation condition of 485 nM. The signal of which can be quickly and reliably captured using a microplate reader.
Species	Human
Source	E. coli
Tag	Tag Free
Accession	Q9UK80 (S209-C564)
Gene ID	27005
Synonyms	USP21; Ubiquitin carboxyl-terminal hydrolase 21; Deubiquitinating enzyme 21; Ubiquitin thioesterase 21; Ubiquitin-specific-processing protease 21
Molecular Weight	40.2 KD
Purity	Greater than 90 % as determined by reducing SDS-PAGE.
Appearance	Solution.
Formulation	Supplied as a 0.2 μm filtered solution of of 50 mM Tris-HCl, 200 mM NaCl, 5% glycerol, 1 mM DTT, pH7.5.
Endotoxin Level	<1 EU/μg, determined by LAL method.
Reconstitution	Please use rapid thawing with running water to thaw the protein.
Storage & Stability	Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping	Shipping with dry ice.
Documentation	Select Batch: Data Sheet (232 KB) SDS (0 KB) COA (205 KB) Handling Instructions (2659 KB)

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Specific Activity (Unit/mg) = 10⁶ ÷ Biological Activity (ED₅₀)

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