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USP3 Protein, Human (Active, sf9, GST)

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USP3 Protein, Human (Active, sf9, GST) is the recombinant human-derived USP3, expressed by sf9 insect cells , with GST labeled tag. ,

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • USP3 Protein, Human (Active, sf9, GST) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

USP3 Protein, Human (Active, sf9, GST) is the recombinant human-derived USP3, expressed by sf9 insect cells , with GST labeled tag. ,

Background

USP3 is a deubiquitinase involved in various cellular processes, including transcriptional regulation, cell cycle progression, and innate immunity. In response to DNA damage, USP3 deubiquitinates monoubiquitinated target proteins such as histone H2A and H2AX, counteracting RNF168- and RNF8-mediated ubiquitination and facilitating the recruitment of DNA damage repair factors to DNA break sites. It is also essential for proper S-phase progression and mitotic entry, and promotes normal cell proliferation and transformation by deubiquitinating and stabilizing TP53. In innate immune memory establishment, USP3 negatively regulates TLR-induced NF-kappa-B signaling by targeting and removing 'Lys-63'-linked polyubiquitin chains on MYD88. Additionally, USP3 suppresses type I interferon signaling by mediating 'Lys-63'-linked polyubiquitin chains on RIGI and IFIH1. Furthermore, USP3 deubiquitinates ASC/PYCARD, the central adapter for inflammasome assembly and activation, thereby promoting inflammasome activation.

Verified Bioactivity

The fundamental role of USP3 is specific removal of ubiquitin from substrates. USP3 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535nM emission light under the excitation condition of 485nM. The signal of which can be quickly and reliably captured using a microplate reader. The concentration of USP3 used is 1.5 nM, and the reaction lasts for 30 minutes. Under these conditions, the signal-to-noise ratio (S/N) of the signal box is greater than or equal to 10.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag N-GST
  • Accession

    Q9Y6I4-1 (E2-L520)

  • Gene ID
    9960
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    USP3; Ubiquitin Specific Peptidase 3; Ubiquitin-Specific-Processing Protease 3; Ubiquitin Carboxyl-Terminal Hydrolase 3; Deubiquitinating Enzyme 3; Ubiquitin Thioesterase 3; Ubiquitin Carboxyl-Terminal Hydrolase; Ubiquitin Specific Protease 3; Ubiquitin T

  • AA Sequence

    ECPHLSSSVCIAPDSAKFPNGSPSSWCCSVCRSNKSPWVCLTCSSVHCGRYVNGHAKKHYEDAQVPLTNHKKSEKQDKVQHTVCMDCSSYSTYCYRCDDFVVNDTKLGLVQKVREHLQNLENSAFTADRHKKRKLLENSTLNSKLLKVNGSTTAICATGLRNLGNTCFMNAILQSLSNIEQFCCYFKELPAVELRNGKTAGRRTYHTRSQGDNNVSLVEEFRKTLCALWQGSQTAFSPESLFYVVWKIMPNFRGYQQQDAHEFMRYLLDHLHLELQGGFNGVSRSAILQENSTLSASNKCCINGASTVVTAIFGGILQNEVNCLICGTESRKFDPFLDLSLDIPSQFRSKRSKNQENGPVCSLRDCLRSFTDLEELDETELYMCHKCKKKQKSTKKFWIQKLPKVLCLHLKRFHWTAYLRNKVDTYVEFPLRGLDMKCYLLEPENSGPESCLYDLAAVVVHHGSGVGSGHYTAYATHEGRWFHFNDSTVTLTDEETVVKAKAYILFYVEHQAKAGSDKL

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • USP3 Protein, Human (Active, sf9, GST) Product Image
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  • USP3 Protein, Human (Active, sf9, GST) Product Image