USP52 Protein, Human (Active, sf9, His, GST)
USP52 Protein, Human (Active, sf9, His, GST) is the recombinant human-derived USP52, expressed by sf9 insect cells , with His, GST labeled tag. ,
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
USP52 Protein, Human (Active, sf9, His, GST) is the recombinant human-derived USP52, expressed by sf9 insect cells , with His, GST labeled tag. ,
Background
USP52 is the catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. It specifically shortens poly(A) tails of RNA, with its activity stimulated by poly(A)-binding protein (PABP). Following PAN deadenylation, the CCR4-NOT complex rapidly degrades the shortened mRNA tails. Deadenylated mRNAs are then degraded via two alternative mechanisms: exosome-mediated 3'-5' exonucleolytic degradation or deadenylation-dependent mRNA decapping followed by 5'-3' exonucleolytic degradation by XRN1. Additionally, USP52 acts as a key regulator of the HIF1A-mediated hypoxic response and is required for HIF1A mRNA stability independently of poly(A) tail length regulation.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag His;GST
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Accession
Q504Q3-1 (M486-R924)
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Gene ID9924
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Protein Length
Partial
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Synonyms
PAN2; HPAN2; Prev. USP52; PAN2 Poly(A) Specific Ribonuclease Subunit Homolog (S. Cerevisiae); KIAA0710; PAB-Dependent Poly(A)-Specific Ribonuclease Subunit PAN2; PAN2-PAN3 Deadenylation Complex Catalytic Subunit PAN2; PAB-Dependent Poly(A)-Specific Ribonu
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AA Sequence
MVSKKYRKVTIKYSKLGLEDFDFKHYNKTLFAGLEPHIPNAYCNCMIQVLYFLEPVRCLIQNHLCQKEFCLACELGFLFHMLDLSRGDPCQGNNFLRAFRTIPEASALGLILADSDEASGKGNLARLIQRWNRFILTQLHQDMQELEIPQAYRGAGGSSFCSSGDSVIGQLFSCEMENCSLCRCGSETVRASSTLLFTLSYPDGSKSDKTGKNYDFAQVLKRSICLDQNTQAWCDTCEKYQPTIQTRNIRHLPDILVINCEVNSSKEADFWRMQAEVAFKMAVKKHGGEISKNKEFALADWKELGSPEGVLVCPSIEELKNVWLPFSIRMKMTKNKGLDVCNWTDGDEMQWGPARAEEEHGVYVYDLMATVVHILDSRTGGSLVAHIKVGETYHQRKEGVTHQQWYLFNDFLIEPIDKHEAVQFDMNWKVPAILYYVKR
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Predicted Molecular Mass
77.8 kDa
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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