Vitamin D-binding protein/GC Protein, Mouse (His)

Vitamin D-binding proteins (GC proteins) play key roles in vitamin D transport and storage and in the clearance of extracellular G-actin. It enhances the C5 alpha chemotactic activity of neutrophils, participates in macrophage activation, and binds to membrane-bound immunoglobulins on B lymphocytes. Vitamin D-binding protein/GC Protein, Mouse (His) is the recombinant mouse-derived Vitamin D-binding protein/GC protein, expressed by E. coli , with N-His labeled tag.

Vitamin D-binding protein (GC Protein) plays a pivotal role in vitamin D transport and storage, contributing to the scavenging of extracellular G-actin. Additionally, it enhances the chemotactic activity of C5 alpha for neutrophils during inflammation and participates in macrophage activation. Notably, GC Protein associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and interacts with IgG Fc receptor on the membranes of T-lymphocytes, suggesting its involvement in immune-related processes. Furthermore, its interaction with LRP2 is crucial for the renal uptake of GC in complex with 25-hydroxyvitamin D3, highlighting its significance in vitamin D metabolism.

Mouse

E. coli

N-His

P21614 (L17-S476)

14473  [NCBI]

Full Length of Mature Protein

LERGRDYEKDKVCNELAMLGKEDFRSLSLILYSRKFSSSTFEQVNQLVKEVVSLTEECCAEGADPTCYDTRTSELSVKSCESDAPFPVHPGTPECCTKEGLERKLCMAALSHQPQEFPTYVEPTNDEICEAFRRDPKGFADQFLYEYSSNYGQAPLPLLVAYTKNYLSMVGSCCTSANPTVCFVKERLQMKHLSLLTTMSNRVCSQYAAYGKEKSRLSHLIKLAQKVPTANLENVLPLAEDFTEILSRCCESTSEDCMASELPEHTIKICQNLSKKNSKFEECCQENTPMNIFMCTYFMPAAEPLQLPAIKLPTGKDLCGQSTTQAMDQYTFELSRRTQVPEVFLSKVLEPTLKTLRECCDTQDSVACFSTQSPLLKRQLTSFIEKGQEMCADYSENTFTEYKKKLAERLRTKTPNTSPAELKDMVEKHSDFASKCCSINSPPLYCSSQIDAEMIDTLQS

Approximately 57.4kDa

• 
  ≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.