Vitamin D-binding protein/GC Protein, Mouse (His)
Based on 1 Customer Validation
Vitamin D-binding proteins (GC proteins) play key roles in vitamin D transport and storage and in the clearance of extracellular G-actin. It enhances the C5 alpha chemotactic activity of neutrophils, participates in macrophage activation, and binds to membrane-bound immunoglobulins on B lymphocytes. Vitamin D-binding protein/GC Protein, Mouse (His) is the recombinant mouse-derived Vitamin D-binding protein/GC protein, expressed by E. coli , with N-His labeled tag.
- Species: Mouse
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
Vitamin D-binding proteins (GC proteins) play key roles in vitamin D transport and storage and in the clearance of extracellular G-actin. It enhances the C5 alpha chemotactic activity of neutrophils, participates in macrophage activation, and binds to membrane-bound immunoglobulins on B lymphocytes. Vitamin D-binding protein/GC Protein, Mouse (His) is the recombinant mouse-derived Vitamin D-binding protein/GC protein, expressed by E. coli , with N-His labeled tag.
Background
Vitamin D-binding protein (GC Protein) plays a pivotal role in vitamin D transport and storage, contributing to the scavenging of extracellular G-actin. Additionally, it enhances the chemotactic activity of C5 alpha for neutrophils during inflammation and participates in macrophage activation. Notably, GC Protein associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and interacts with IgG Fc receptor on the membranes of T-lymphocytes, suggesting its involvement in immune-related processes. Furthermore, its interaction with LRP2 is crucial for the renal uptake of GC in complex with 25-hydroxyvitamin D3, highlighting its significance in vitamin D metabolism.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Mouse
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Source E. coli
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Tag N-His
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Accession
P21614 (L17-S476)
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Molecular Construction
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N-term
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His
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GC (L17-S476)
Accession # P21614 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
GC; GcMAF; GC Vitamin D Binding Protein; HDBP; DBP; VDB; Vitamin D-Binding Protein; Epididymis Secretory Protein Li 51; Gc-Globulin; Vitamin D-Binding Alpha-Globulin; VDBP; GC, Vitamin D Binding Protein; Vitamin D-Binding Protein-Macrophage Activating Fac
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AA Sequence
LERGRDYEKDKVCNELAMLGKEDFRSLSLILYSRKFSSSTFEQVNQLVKEVVSLTEECCAEGADPTCYDTRTSELSVKSCESDAPFPVHPGTPECCTKEGLERKLCMAALSHQPQEFPTYVEPTNDEICEAFRRDPKGFADQFLYEYSSNYGQAPLPLLVAYTKNYLSMVGSCCTSANPTVCFVKERLQMKHLSLLTTMSNRVCSQYAAYGKEKSRLSHLIKLAQKVPTANLENVLPLAEDFTEILSRCCESTSEDCMASELPEHTIKICQNLSKKNSKFEECCQENTPMNIFMCTYFMPAAEPLQLPAIKLPTGKDLCGQSTTQAMDQYTFELSRRTQVPEVFLSKVLEPTLKTLRECCDTQDSVACFSTQSPLLKRQLTSFIEKGQEMCADYSENTFTEYKKKLAERLRTKTPNTSPAELKDMVEKHSDFASKCCSINSPPLYCSSQIDAEMIDTLQS
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Predicted Molecular Mass
57.4 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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