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MEP50/WDR77 Protein, Human (His-SUMO)

WDR77 is a non-catalytic component of the methylosome complex with PRMT5 and CLNS1A and regulates arginine to dimethylarginine in spliceosomal Sm proteins and histones. This modification directs Sm proteins to SMN complexes, helping them assemble into small nuclear ribonucleoprotein core particles. WDR77 Protein, Human (His-SUMO) is the recombinant human-derived WDR77 protein, expressed by E. coli , with N-His, N-SUMO labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • MEP50/WDR77 Protein, Human (His-SUMO) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

WDR77 is a non-catalytic component of the methylosome complex with PRMT5 and CLNS1A and regulates arginine to dimethylarginine in spliceosomal Sm proteins and histones. This modification directs Sm proteins to SMN complexes, helping them assemble into small nuclear ribonucleoprotein core particles. WDR77 Protein, Human (His-SUMO) is the recombinant human-derived WDR77 protein, expressed by E. coli , with N-His, N-SUMO labeled tag.

Background

WDR77 functions as a non-catalytic component within the methylosome complex, alongside PRMT5 and CLNS1A, orchestrating the modification of specific arginines to dimethylarginines in spliceosomal Sm proteins and histones. This modification directs Sm proteins to the survival of motor neurons (SMN) complex, facilitating their assembly into small nuclear ribonucleoprotein core particles. Beyond its role in RNA processing, WDR77 may play a part in transcription regulation. The methylosome complex extends its methylating activity to Piwi proteins (PIWIL1, PIWIL2, and PIWIL4), essential for their interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. WDR77 engages in a complex interplay with other components such as PRMT1, ERH, and CLNS1A, with specific interactions observed with PRMT5, various Sm proteins, SUZ12, histone H2A, CTDP1, LSM11, APEX1, AR, NKX3-1, CHTOP, FAM47E, and TSC22D2. Its intricate network of interactions underscores WDR77's multifaceted involvement in epigenetic modifications and regulatory pathways.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-His;N-SUMO
  • Accession

    Q9BQA1 (M1-L312)

  • Gene ID
    79084  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His-SUMO
    • WDR77 (M1-L312)
      Accession # Q9BQA1
    • C-term
  • Protein Length

    Partial

  • Synonyms

    WDR77; WD Repeat Domain 77; Methylosome Protein 50; MEP50; WD Repeat-Containing Protein 77; Methylosome Protein WDR77; P44; Androgen Receptor Cofactor P44; P44/Mep50; MEP-50; Testis Tissue Sperm-Binding Protein Li 44a; Nbla10071; HKMT1069; WD45

  • AA Sequence

    MRKETPPPLVPPAAREWNLPPNAPACMERQLEAARYRSDGALLLGASSLSGRCWAGSLWLFKDPCAAPNEGFCSAGVQTEAGVADLTWVGERGILVASDSGAVELWELDENETLIVSKFCKYEHDDIVSTVSVLSSGTQAVSGSKDICIKVWDLAQQVVLSSYRAHAAQVTCVAASPHKDSVFLSCSEDNRILLWDTRCPKPASQIGCSAPGYLPTSLAWHPQQSEVFVFGDENGTVSLVDTKSTSCVLSSAVHSQCVTGLVFSPHSVPFLASLSEDCSLAVLDSSLSELFRSQAHRDFVRDATWSPLNHSL

  • Predicted Molecular Mass

    49.6 kDa

  • Molecular Weight

    Approximately 51 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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  • MEP50/WDR77 Protein, Human (His-SUMO) Product Image