YUAB Protein, Bacillus subtilis (Cell-Free, His-SUMO)
YUAB is an important contributor to biofilm formation, self-polymerizing to form a hydrophobic layer with excellent stability and resistance to mechanical compression. YUAB is essential for complex colony structure, interacting synergistically with exopolysaccharides and TasA amyloid fibrils to facilitate biofilm matrix assembly. YUAB Protein, Bacillus subtilis (Cell-Free, His-SUMO) is the recombinant YUAB protein, expressed by E. coli Cell-free , with N-His, N-SUMO labeled tag.
- Species: Others
- Source: E. coli Cell-free
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
YUAB is an important contributor to biofilm formation, self-polymerizing to form a hydrophobic layer with excellent stability and resistance to mechanical compression. YUAB is essential for complex colony structure, interacting synergistically with exopolysaccharides and TasA amyloid fibrils to facilitate biofilm matrix assembly. YUAB Protein, Bacillus subtilis (Cell-Free, His-SUMO) is the recombinant YUAB protein, expressed by E. coli Cell-free , with N-His, N-SUMO labeled tag.
Background
YUAB, a key player in biofilm formation, engages in self-polymerization, creating a distinctive layer on the biofilm surface that imparts hydrophobic properties. This layer demonstrates stability and resilience, displaying resistance to significant mechanical force compression. Crucial for the development of complex colony architecture, YUAB is implicated in synergistic interactions with exopolysaccharides and TasA amyloid fibers, facilitating the assembly of the biofilm matrix. As a polymer-forming entity, YUAB contributes to the structural integrity and hydrophobic characteristics of the biofilm.
Technical Parameters
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Species Others
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Source E. coli Cell-free
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Tag N-His;N-SUMO
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Accession
P71014 (M1-N181)
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Gene ID938831 [NCBI]
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Molecular Construction
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N-term
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6*His-SUMO
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YUAB (M1-N181)
Accession # P71014 -
C-term
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Protein Length
Full Length
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Synonyms
Biofilm-surface layer protein A; ORF-1; bslA; yuaB; BSU31080
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AA Sequence
MKRKLLSSLAISALSLGLLVSAPTASFAAESTSTKAHTESTMRTQSTASLFATITGASKTEWSFSDIELTYRPNTLLSLGVMEFTLPSGFTANTKDTLNGNALRTTQILNNGKTVRVPLALDLLGAGEFKLKLNNKTLPAAGTYTFRAENKSLSIGNKFYAEASIDVAKRSTPPTQPCGCN
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Predicted Molecular Mass
35.3 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US;may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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