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YUAB Protein, Bacillus subtilis (Cell-Free, His-SUMO)

Cat. No.: HY-P72009
Handling Instructions

YUAB is an important contributor to biofilm formation, self-polymerizing to form a hydrophobic layer with excellent stability and resistance to mechanical compression. YUAB is essential for complex colony structure, interacting synergistically with exopolysaccharides and TasA amyloid fibrils to facilitate biofilm matrix assembly. YUAB Protein, Bacillus subtilis (Cell-Free, His-SUMO) is the recombinant YUAB protein, expressed by E. coli Cell-free , with N-His, N-SUMO labeled tag. The total length of YUAB Protein, Bacillus subtilis (Cell-Free, His-SUMO) is 181 a.a., with molecular weight of ~35.3 kDa.

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Description

YUAB is an important contributor to biofilm formation, self-polymerizing to form a hydrophobic layer with excellent stability and resistance to mechanical compression. YUAB is essential for complex colony structure, interacting synergistically with exopolysaccharides and TasA amyloid fibrils to facilitate biofilm matrix assembly. YUAB Protein, Bacillus subtilis (Cell-Free, His-SUMO) is the recombinant YUAB protein, expressed by E. coli Cell-free , with N-His, N-SUMO labeled tag. The total length of YUAB Protein, Bacillus subtilis (Cell-Free, His-SUMO) is 181 a.a., with molecular weight of ~35.3 kDa.

Background

YUAB, a key player in biofilm formation, engages in self-polymerization, creating a distinctive layer on the biofilm surface that imparts hydrophobic properties. This layer demonstrates stability and resilience, displaying resistance to significant mechanical force compression. Crucial for the development of complex colony architecture, YUAB is implicated in synergistic interactions with exopolysaccharides and TasA amyloid fibers, facilitating the assembly of the biofilm matrix. As a polymer-forming entity, YUAB contributes to the structural integrity and hydrophobic characteristics of the biofilm.

Species

Others

Source

E. coli Cell-free

Tag

N-His;N-SUMO

Accession

P71014 (M1-N181)

Gene ID

938831  [NCBI]

Molecular Construction
N-term
6*His-SUMO
YUAB (M1-N181)
Accession # P71014
C-term
Synonyms
bslA; yuaB; BSU31080Biofilm-surface layer protein A; ORF-1
AA Sequence

MKRKLLSSLAISALSLGLLVSAPTASFAAESTSTKAHTESTMRTQSTASLFATITGASKTEWSFSDIELTYRPNTLLSLGVMEFTLPSGFTANTKDTLNGNALRTTQILNNGKTVRVPLALDLLGAGEFKLKLNNKTLPAAGTYTFRAENKSLSIGNKFYAEASIDVAKRSTPPTQPCGCN

Molecular Weight

Approximately 35.3 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

YUAB Protein, Bacillus subtilis (Cell-Free, His-SUMO) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
YUAB Protein, Bacillus subtilis (Cell-Free, His-SUMO)
Cat. No.:
HY-P72009
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