ZBED1 Protein, Human (His)

As an E3-type SUMO ligase, ZBED1 protein catalyzes CHD3/Mi2-α sumoylation, relieves transcriptional repression and promotes the recruitment of RNA polymerase II to gene promoters. This positively regulates H1-5, RPS6, RPL10A and RPL12 transcription, enhancing cell proliferation and growth. ZBED1 Protein, Human (His) is the recombinant human-derived ZBED1 protein, expressed by E. coli , with C-6*His labeled tag. The total length of ZBED1 Protein, Human (His) is 98 a.a., with molecular weight of ~14.0 kDa.

The ZBED1 protein functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, catalyzing the sumoylation of CHD3/Mi2-alpha, leading to its dissociation from DNA and subsequent relief of transcriptional repression. This event enhances the recruitment of RNA polymerase II to gene promoters, positively regulating the transcription of various genes, including H1-5 and ribosomal proteins like RPS6, RPL10A, and RPL12. The heightened transcriptional activity orchestrated by ZBED1 promotes cell proliferation, contributing to cellular growth. ZBED1 specifically binds to 5'-TGTCG[CT]GA[CT]A-3' consensus sequences in the gene promoters of ribosomal proteins . In the context of microbial infection, ZBED1 also binds to human adenovirus gene promoters, playing a role in transcriptional repression and impeding virus growth during the early stages of infection.

Human

E. coli

C-6*His

O96006 (N3-E100)

9189  [NCBI]

NKSLESSQTDLKLVAHPRAKSKVWKYFGFDTNAEGCILQWKKIYCRICMAQIAYSGNTSNLSYHLEKNHPEEFCEFVKSNTEQMREAFATAFSKLKPE

Approximately 14.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.