ZBED1 Protein, Human (His)
Based on 1 Customer Validation
As an E3-type SUMO ligase, ZBED1 protein catalyzes CHD3/Mi2-α sumoylation, relieves transcriptional repression and promotes the recruitment of RNA polymerase II to gene promoters. This positively regulates H1-5, RPS6, RPL10A and RPL12 transcription, enhancing cell proliferation and growth. ZBED1 Protein, Human (His) is the recombinant human-derived ZBED1 protein, expressed by E. coli , with C-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
As an E3-type SUMO ligase, ZBED1 protein catalyzes CHD3/Mi2-α sumoylation, relieves transcriptional repression and promotes the recruitment of RNA polymerase II to gene promoters. This positively regulates H1-5, RPS6, RPL10A and RPL12 transcription, enhancing cell proliferation and growth. ZBED1 Protein, Human (His) is the recombinant human-derived ZBED1 protein, expressed by E. coli , with C-6*His labeled tag.
The ZBED1 protein functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, catalyzing the sumoylation of CHD3/Mi2-alpha, leading to its dissociation from DNA and subsequent relief of transcriptional repression. This event enhances the recruitment of RNA polymerase II to gene promoters, positively regulating the transcription of various genes, including H1-5 and ribosomal proteins like RPS6, RPL10A, and RPL12. The heightened transcriptional activity orchestrated by ZBED1 promotes cell proliferation, contributing to cellular growth. ZBED1 specifically binds to 5'-TGTCG[CT]GA[CT]A-3' consensus sequences in the gene promoters of ribosomal proteins . In the context of microbial infection, ZBED1 also binds to human adenovirus gene promoters, playing a role in transcriptional repression and impeding virus growth during the early stages of infection.
Technical Parameters
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Species Human
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Source E. coli
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Tag C-6*His
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Accession
O96006 (N3-E100)
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Molecular Construction
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N-term
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ZBED1 (N3-E100)
Accession # O96006 -
6*His
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C-term
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Protein Length
Partial
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Synonyms
ZBED1; Zinc Finger BED‑Type Containing 1; ALTE; TRAMP; HDREF; DREF; KIAA0785; Zinc Finger BED Domain‑Containing Protein 1; Ac‑Like Transposable Element; E3 SUMO‑Protein Ligase ZBED1; DREF Homolog; BED‑Type Zinc Finger Domain‑Containing Protein 1; DNA Repl
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AA Sequence
NKSLESSQTDLKLVAHPRAKSKVWKYFGFDTNAEGCILQWKKIYCRICMAQIAYSGNTSNLSYHLEKNHPEEFCEFVKSNTEQMREAFATAFSKLKPE
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Predicted Molecular Mass
12.5 kDa
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Molecular Weight
Approximately 14 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (235 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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