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ZP3 Protein, Human (HEK293, His-Myc)

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Based on 1 Customer Validation

ZP3 protein, a vital component of the zona pellucida, is crucial for sperm binding, inducing the acrosome reaction, and preventing polyspermy. Alongside ZP1, ZP2, and ZP4, it forms a glycoprotein complex in the zona matrix, organizing into filaments cross-linked by ZP1 homodimers. ZP3 actively interacts with ZP1 and ZP2, highlighting its integral role in the molecular architecture governing fertilization processes. ZP3 Protein, Human (HEK293, His-Myc) is the recombinant human-derived ZP3 protein, expressed by HEK293 , with N-His, N-Myc labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for ZP3 Protein, Human (HEK293, His-Myc)
  • ZP3 Protein, Human (HEK293, His-Myc) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

ZP3 protein, a vital component of the zona pellucida, is crucial for sperm binding, inducing the acrosome reaction, and preventing polyspermy. Alongside ZP1, ZP2, and ZP4, it forms a glycoprotein complex in the zona matrix, organizing into filaments cross-linked by ZP1 homodimers. ZP3 actively interacts with ZP1 and ZP2, highlighting its integral role in the molecular architecture governing fertilization processes. ZP3 Protein, Human (HEK293, His-Myc) is the recombinant human-derived ZP3 protein, expressed by HEK293 , with N-His, N-Myc labeled tag.

Background

As a crucial element of the zona pellucida, ZP3 protein contributes to the extracellular matrix surrounding oocytes, playing a pivotal role in mediating sperm binding, inducing the acrosome reaction, and preventing post-fertilization polyspermy. Within the zona pellucida, ZP3, along with ZP1, ZP2, and ZP4, forms a glycoprotein complex essential for sperm binding and the formation of the zona matrix. ZP2 and ZP3 polymers organize into long filaments cross-linked by ZP1 homodimers, highlighting the structural intricacies of the zona pellucida matrix. Moreover, ZP3 actively interacts with ZP1 and ZP2, underscoring its integral role in the complex molecular architecture that governs fertilization processes.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag N-6*His;N-Myc
  • Accession

    P21754 (Q23-V387)

  • Gene ID
    7784  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His-Myc
    • ZP3 (Q23-V387)
      Accession # P21754
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    ZP3; ZP3-424; Prev. ZP3A; Zp-3; Prev. ZP3B; Zona Pellucida Glycoprotein 3A (Sperm Receptor); ZPC; Zona Pellucida Glycoprotein ZP3; Zona Pellucida Sperm-Binding Protein 3; Zona Pellucida Glycoprotein 3B; Zona Pellucida Protein C; OZEMA3; Sperm Receptor; OO

  • AA Sequence

    QPLWLLQGGASHPETSVQPVLVECQEATLMVMVSKDLFGTGKLIRAADLTLGPEACEPLVSMDTEDVVRFEVGLHECGNSMQVTDDALVYSTFLLHDPRPVGNLSIVRTNRAEIPIECRYPRQGNVSSQAILPTWLPFRTTVFSEEKLTFSLRLMEENWNAEKRSPTFHLGDAAHLQAEIHTGSHVPLRLFVDHCVATPTPDQNASPYHTIVDFHGCLVDGLTDASSAFKVPRPGPDTLQFTVDVFHFANDSRNMIYITCHLKVTLAEQDPDELNKACSFSKPSNSWFPVEGSADICQCCNKGDCGTPSHSRRQPHVMSQWSRSASRNRRHVTEEADVTVGPLIFLDRRGDHEVEQWALPSDTSV

  • Predicted Molecular Mass

    41.7 kDa

  • Molecular Weight

    Approximately 55-75 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 7.4, 20% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • ZP3 Protein, Human (HEK293, His-Myc) Product Image
    1/16
  • Experimental Validation Results for ZP3 Protein, Human (HEK293, His-Myc)
  • ZP3 Protein, Human (HEK293, His-Myc) Product Image