RIPK1-IN-32
RIPK1-IN-32 is a RIPK inhibitor with anti-inflammatory activity. RIPK1-IN-32 inhibits nitric oxide (NO) release with an IC50 of 3.26 μM. RIPK1-IN-32 significantly alleviates acute liver injury associated with sepsis through the RIPK1/NF-κB/MAPK pathway, therefore preventing the nuclear translocation of p65 and c-fos, which results in reduced expression of TNF-α and IL-6. RIPK1-IN-32 can be used for the study of acute liver injury and sepsis.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 分子式: C42H59NO7S2
- 分子量:754.05
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
IC50 & Target
[1]|
NF-κB |
IL-6 |
RIPK1 |
体外実験
RIPK1-IN-32 (Compound 12) (0-25 μM, 45-63 °C) significantly inhibits the thermal degradation of RIPK1 in RAW264.7 macrophages[1].
RIPK1-IN-32 (2.5 μM) significantly diminishes p65 and c-fos nuclear translocation in RAW264.7 macrophages[1].
RIPK1-IN-32 (0.625-2.5 μM, 8 h) notably reduces d the levels of IL-6 and TNF-α in RAW 264.7 cells[1].
RIPK1-IN-32 (0.625-2.5 μM, 8 h) effectively hinders the LPS (HY-D1056B3)-induced activation of the RIPK1/NF-κB/MAPK pathway[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:LPS-stimulated RAW 264.7 macrophages
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Concentration:0.625, 1.25, 2.5 μM
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Incubation Time:8 h, including 1 μg/mL LPS for 6 hours
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Result:Significantly diminished the contents of IL-6 and TNF-α.
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Cell Line:LPS-stimulated RAW 264.7 macrophages
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Concentration:0.625, 1.25, 2.5 μM
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Incubation Time:8 h, including 1 μg/mL LPS for 6 hours
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Result:Resulted in notable decreases in the levels of RIPK1, p-p65, and p-IκB suggesting an inhibition of NF-κB signaling.
Found to lower the protein levels of p-p38, p-ERK, and p-JNK which aligns with the suppression of MAPK signaling.
体内実験
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:LPS injection into the tail vein to create a sepsis model established in Male BALB/c mice (18-20 g)[1]
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Dosage:1.25, 2.5 and 5 mg/kg
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Administration:Intravenous injection (i.v.), single dose
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Result:Markedly alleviated LPS-induced ALI.
Decreased the serum levels of ALT and AST.
Significantly diminished the contents of IL-6 and TNF-α.
Attenuated the damage to lung tissue and markedly lessened the pathological alterations noted in the kidneys subsequent.
Decreased in liver concentrations of RIPK1, p-p65, P-IκB, p-p38, p-ERK, and p-JNK.
化学情報
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分子量 754.05
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分子式 C42H59NO7S2
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SMILES
[H][C@@]12CC[C@@]([C@]3(C)[C@@](C4=C(C(O[C@H]4C3)=O)CC/C=C(C)/C)([H])C[C@H]5O)(C)[C@@]5([C@]1(CC[C@H]([C@H]2C)OC(CCSSCCC(NOCC6=CC=CC=C6)=O)=O)C)[H]
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
RIPK1-IN-32 関連分類
HY-175007 関連分類
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